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European Food Research and Technology

, Volume 235, Issue 2, pp 253–263 | Cite as

A rapid PCR–RFLP method for the identification of Lophius species

  • Andrea ArmaniEmail author
  • Lorenzo Castigliego
  • Lara Tinacci
  • Gabriele Gandini
  • Daniela Gianfaldoni
  • Alessandra Guidi
Original Paper

Abstract

The seven Anglerfish species, which belong to the genus Lophius, have a different value on the market, worldwide. If whole fishes can be identified by their morphological characteristics, they become indistinguishable when prepared or processed. In this study, a rapid method based on polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) was developed for the authentication of the seven Lophius species, using a cytochrome b gene fragment of 566 bp. After a genus-specific PCR, a fast digestion with the restriction enzyme BfaI, followed by agarose gel electrophoresis, allowed a clear species identification by producing specific restriction patterns. The total time required was as low as 6 h, DNA extraction included. The method was then used to analyse 48 commercial samples, whose phylogenetic analysis confirmed the PCR–RFLP response at 100 %. Results showed that mislabelling occurs on the market regardless the kind of processing.

Keywords

PCR–RFLP Species identification Lophius Cytochrome b 

Notes

Acknowledgments

The authors wish to thank Dr. Paolo Manzoni for his help in species identifications.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Andrea Armani
    • 1
    Email author
  • Lorenzo Castigliego
    • 1
  • Lara Tinacci
    • 1
  • Gabriele Gandini
    • 2
  • Daniela Gianfaldoni
    • 1
  • Alessandra Guidi
    • 1
  1. 1.Department of Animal Pathology, Prophylaxis and Food HygieneUniversity of PisaPisaItaly
  2. 2.Ministry of Health DGSAN UVAC Veneto-PIFVenetoItaly

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