European Food Research and Technology

, Volume 234, Issue 5, pp 821–832 | Cite as

Cinnamon essential oil and cinnamaldehyde in the control of bacterial biofilms formed on stainless steel surfaces

  • Maíra Maciel Mattos de OliveiraEmail author
  • Danilo Florisvaldo Brugnera
  • Josianne Arantes do Nascimento
  • Nádia Nara Batista
  • Roberta Hilsdorf Piccoli
Original Paper


The antibacterial effects of Cinnamomum cassia essential oil (EO) and cinnamaldehyde were evaluated against single- and mixed-species cultivation of enteropathogenic Escherichia coli (EPEC) and Listeria monocytogenes attached to stainless steel. A central composite rotational design with two variables and eleven assays was used to optimize the concentrations (0.00–1.00% v/v for the EO and 0.00–0.80% v/v for cinnamaldehyde) and contact times (1–21 min). The models generated were validated, and the effectiveness of C. cassia EO and cinnamaldehyde was compared with that of commercially available chemical sanitizers. Cinnamaldehyde and C. cassia EO proved to be efficient alternatives to commercial chemical sanitizers in the reduction or elimination of sessile bacterial cells. The activity of these natural compounds was, in most cases, equivalent or superior to that of the chemical sanitizers tested. However, L. monocytogenes was more resistant than EPEC to C. cassia EO and cinnamaldehyde, and the bacterial association in mixed-species biofilms made them more susceptible to these compounds.


Food-borne pathogens Enteropathogenic Escherichia coli (EPEC) Listeria monocytogenes Bacterial adhesion Natural sanitizers 



The authors would like to thank the National Council of Technological and Scientific Development (CNPq) for scholarships to the first and second authors and the Research Support Foundation of the State of Minas Gerais (FAPEMIG) for financial support.


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Maíra Maciel Mattos de Oliveira
    • 1
    Email author
  • Danilo Florisvaldo Brugnera
    • 1
  • Josianne Arantes do Nascimento
    • 1
  • Nádia Nara Batista
    • 1
  • Roberta Hilsdorf Piccoli
    • 1
  1. 1.Department of Food ScienceFederal University of LavrasLavrasBrazil

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