European Food Research and Technology

, Volume 233, Issue 6, pp 923–930 | Cite as

Folate content in Swedish eggs: influence of breed, feed and processing

  • Hanna Sara Strandler
  • Jelena Jastrebova
  • Irene Mattisson
Original Paper


Folate content in eggs from three different breeds of hens fed with three different feeds, one organic and two conventional, was compared using a simplified microbiological assay. Retention of folate when boiling the eggs was also studied. Folate content in eggs did not differ significantly due to the breed of hen or the feed used. The loss of folate when boiling the eggs was 9%. The results from the microbiological assay were confirmed by LC/MS. According to this study, 1 egg (60 g) provides 40–50 μg of folate. This corresponds to 13–17% of the recommended daily intake, 300 μg for adults in the Nordic Nutrition Recommendations. The folate forms found in egg yolk with LC/MS were 5-methyl-tetrahydrofolate and 10-formyl-folic acid, whereas no folates were found in egg white. In this study, no polyglutamate folate forms could be detected in eggs.


Folate Food analysis HPLC  LC/MS Microbiological assay Egg Egg yolk 



We are very grateful to Professor Margaretha Jägerstad for her interest in the study and for valuable discussions on the manuscript and to Johan Patring for supervising the LC/MS analysis. The gifts of folic acid and folate standards from Merck Eprova AG, Schaffhausen, Switzerland are gratefully acknowledged.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Hanna Sara Strandler
    • 1
  • Jelena Jastrebova
    • 2
  • Irene Mattisson
    • 1
  1. 1.The National Food AdministrationUppsalaSweden
  2. 2.Department of Food ScienceSwedish University of Agricultural SciencesUppsalaSweden

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