European Food Research and Technology

, Volume 233, Issue 6, pp 915–922

Effect of angiotensin I-converting enzyme (ACE) inhibitory peptide purified from enzymatic hydrolysates of Styela plicata

  • Seok-Chun Ko
  • Min Cheol Kang
  • Jung-Kwon Lee
  • Hee-Guk Byun
  • Se-Kwon Kim
  • Seung-Cheol Lee
  • Byong-Tae Jeon
  • Pyo-Jam Park
  • Won-Kyo Jung
  • You-Jin Jeon
Original paper

DOI: 10.1007/s00217-011-1585-7

Cite this article as:
Ko, SC., Kang, M.C., Lee, JK. et al. Eur Food Res Technol (2011) 233: 915. doi:10.1007/s00217-011-1585-7

Abstract

Angiotensin I-converting enzyme (ACE) inhibitory peptide was isolated from Styela plicata. The S. plicata was hydrolyzed with various proteases including Protamex, Kojizyme, Neutrase, Flavourzyme, Alcalase, trypsin, α-chymotrypsin, pepsin, and papain. The hydrolysate prepared with Protamex had the highest ACE inhibitory activity compared to the other hydrolysates. We attempted to isolate ACE inhibitory peptides from hydrolysate prepared with Protamex using ultra-filtration, gel filtration on a Sephadex G-25 column and reversed-phase high-performance liquid chromatography (RP-HPLC) on an ODS column. IC50 value of the purified ACE inhibitory peptide was 24.7 μM, and Lineweaver–Burk plots suggest that the purified peptide from S. plicata acts as mixed-type inhibitor against ACE. Amino acid sequence of the purified peptide was identified as Met-Leu-Leu-Cys-Ser, with a molecular weight 566.4 Da. The results of this study suggest that peptides derived from S. plicata may be beneficial as anti-hypertension compounds in functional foods resource.

Keywords

Styela plicata ACE inhibitory peptide Enzymatic hydrolysis Purification 

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Seok-Chun Ko
    • 1
  • Min Cheol Kang
    • 1
  • Jung-Kwon Lee
    • 2
  • Hee-Guk Byun
    • 2
  • Se-Kwon Kim
    • 3
  • Seung-Cheol Lee
    • 4
  • Byong-Tae Jeon
    • 5
  • Pyo-Jam Park
    • 6
  • Won-Kyo Jung
    • 7
  • You-Jin Jeon
    • 1
  1. 1.Department of Marine Life ScienceJeju National UniversityJejuRepublic of Korea
  2. 2.Faculty of Marine Bioscience and TechnologyKangnung-Wonju National UniversityGangneungRepublic of Korea
  3. 3.Department of ChemistryPukyoung National UniversityBusanRepublic of Korea
  4. 4.Division of Food Science and TechnologyKyungnam UniversityMasanRepublic of Korea
  5. 5.Korean Nokyong Research Center, College of MedicineKonkuk UniversityChungjuRepublic of Korea
  6. 6.Department of Biotechnology, College of MedicineKonkuk UniversityChungjuRepublic of Korea
  7. 7.Department of Marine Life Science, Marine Life Research CenterChosun UniversityGwangjuRepublic of Korea

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