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European Food Research and Technology

, Volume 233, Issue 1, pp 11–18 | Cite as

Antioxidant activity of rosemary and thyme by-products and synergism with added antioxidant in a liposome system

  • Gema NietoEmail author
  • Kevin Huvaere
  • Leif H. Skibsted
Original Paper

Abstract

This study describes the use of liposomes as biological membrane models to evaluate the potential of natural antioxidants as inhibitors of lipid peroxidation. Antioxidative effects of by-products from manufacturing of essential oils, i.e., distilled rosemary leaf residues (DRL), distilled thyme leaf residues (DTL), and the combined antioxidative effects of DRL or DTL with α-tocopherol (TOH), ascorbic acid (AA), and quercetin (QC) on peroxidation of l-α-phosphatidylcholine liposomes as initiated by hydrophilic azo-initiators, were investigated. In addition, experiments were repeated with whole thyme leaves (TL) to compare the characteristics of by-products (leaf residues from distillation) with the initial industrial product (leaf for distillation). Extracts from DRL, DTL, and TL all had an obvious antioxidative effect as evidenced by a lag phase for the formation of phosphatidylcholine-derived conjugated dienes. DRL and DTL had similar antioxidative activity, while whole thyme leaves showed superior antioxidant activity compared with distilled thyme residues. Combination of TOH or QC with DRL, DTL, and TL, respectively, showed synergism in prolonging of the lag phase.

Keywords

Thymus zygis Rosmarinus officinalis Liposomes Lipid oxidation Antioxidants By-products 

Notes

Acknowledgments

The authors are grateful to the University of Murcia and Fundación Séneca (Agencia de Ciencia y Tecnología de la Region de Murcia) for the postdoctoral fellowship of Gema Nieto (12494/PD/09).

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Department of Food Technology and Human Nutrition, Veterinary FacultyUniversity of MurciaMurciaSpain
  2. 2.Food Chemistry, Department of Food Science, Faculty of Life SciencesUniversity of CopenhagenFrederiksberg CDenmark

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