European Food Research and Technology

, Volume 232, Issue 6, pp 979–984 | Cite as

A nondestructive method for estimating freshness of freshwater fish

Original Paper


The traditional indictors of fish freshness including total aerobic count (TAC), K value, total volatile basic nitrogen (TVB-N), and sensory assessment (SA) were measured regularly to analyze the freshness changes during chill storage. Electric conduction property of whole fish was also studied by measuring their impedances under different frequencies as a fast nondestructive method. The relationship between traditional freshness indictors and electric conductivity was analyzed to determine the feasibility of evaluating fish freshness based on impedance change ratio (Q value) during storage. The results showed that traditional freshness indictors (TAC, K value, and TVB-N) values increased as storage time prolonged, while Q value and SA decreased. There were good relationships between Q value and TAC, K value, TVB-N, and SA (P < 0.01), with the correlation coefficients were 0.943, 0.996, 0.951, and 0.968, respectively. Thus, Q value can be used as a valid index for freshness evaluation and this method via measuring the electric conductivity property of whole fish is a fast nondestructive method for determining the freshness of fish during storage.


Electric impedance Freshness Grass carp Total aerobic count Total volatile basic nitrogen K value Sensory assessment 



This study was supported by the earmarked fund for China Agriculture Research System (CARS-46).


  1. 1.
    Olafsdottir G, Martinsdottir E, Oehlenschlager J, Dalgaarg P, Undeland I, Mackie IM et al (1997) Method to evaluate fish freshness in research and industry. Trends Food Sci Technol 8:258–265CrossRefGoogle Scholar
  2. 2.
    Grigorakis K (2007) Compositional and organoleptic quality of farmed and wild gilthead sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax) and factors affecting it: a review. Aquaculture 272:55–75CrossRefGoogle Scholar
  3. 3.
    Bremner HA, Sakaguchi M (2000) A critical look at whether ‘freshness’ can be determined. J Aquat Food Pro Technol 9:5–25CrossRefGoogle Scholar
  4. 4.
    Baixas-Nogueras S, Bover-Cid S, Veciana-Nogueäs T, Vidal-Carou MC (2002) Chemical and sensory changes in mediterranean hake (Merluccius merluccius) under refrigeration (6–8 °C) and stored in Ice. J Agric Food Chem 50:6504–6510CrossRefGoogle Scholar
  5. 5.
    Campus M, Addis MF, Cappuccinelli R, Porcu MC, Pretti L, Tedde V, Secchi N, Stara G, Roggio T (2010) Stress relaxation behaviour and structural changes of muscle tissues from Gilthead Sea Bream (Sparus aurata L.) following high pressure treatment. J Food Eng 96:192–198CrossRefGoogle Scholar
  6. 6.
    Cao R, Xue CH, Liu Q, Yin BZ (2009) Microbiological, chemical and sensory assessment of (I) whole ungutted, (II) whole gutted and (III) filleted tilapia (Oreochromis niloticus) during refrigerated storage. Int J Food Sci Technol 44:2243–2248CrossRefGoogle Scholar
  7. 7.
    Barat JM, Gill L, García-Breijo E, Aristoy MC, Toldrá F, Martíneai -Mañez R, Soto J (2008) Freshness monitoring of sea bream with a potentiometric sensor. Food Chem 108:681–688CrossRefGoogle Scholar
  8. 8.
    Barsoukov E, Macdonald J, Ross (eds) (2005) Impedance spectroscropy theory, experiment, and applications, 2nd edn. Wiley, HobokenGoogle Scholar
  9. 9.
    Damez JL, Clerjon S (2008) Meat quality assessment using biophysical methods related to meat structure. Meat Sci 80:132–149CrossRefGoogle Scholar
  10. 10.
    Jukna V, Jukna Č, Pečiulaitiene N, Kerinas E (2009) Influence of cattle sex and age on carcass yield and muscularity class Galviju{ogonek} lyties ir amžiaus itaka skerdenu{ogonek} išeigai ir raumeningumo klasei. Veterinarija ir Zootechnika 46:20–23Google Scholar
  11. 11.
    Kleinová I, Ingr I (1999) Dynamics of pH and electric conductivity values in longissimus and semimembranosus muscles of pigs [Vývoj hodnot pH a elektrické vodivosti ve vepřovém mase v průběhu zrání]. Czech J Anim Sci 44:551–554Google Scholar
  12. 12.
    Tejada M, Heras CDL, Kent M (2007) Changes in the quality indices during ice storage of farmed Senegalese sole (Solea senegalensis). Eur Food Res Technol 225:225–232CrossRefGoogle Scholar
  13. 13.
    Niu J, Lee JY (2000) A new approach for the determination of fish freshness by electrochemical impedance spectroscopy. J Food Sci 65:780–785CrossRefGoogle Scholar
  14. 14.
    Rümenappa C, Remm M, Wolf B, Gleich B (2009) Improved method for impedance measurements of mammalian cells. Biosens Bioelectron 24:2915–2919CrossRefGoogle Scholar
  15. 15.
    Zhang LN, Shen HX, Luo YK (2010) Study on the electric conduction properties of fresh and frozen-thawed grass carp (Ctenopharyngodon idellus) and tilapia (Oreochromis niloticus). Int J Food Sci Technol 45:2560–2564CrossRefGoogle Scholar
  16. 16.
    AOAC (2002) Official methods of analysis, 17th edn. Association of Official Analytical Chemists, Washington, DCGoogle Scholar
  17. 17.
    Song YL, Liu L, Shen HX, You J, Luo YK (2011) Effect of sodium alginate-based edible coating containing different anti-oxidants on quality and shelf life of refrigerated bream (Megalobrama amblycephala). Food Control 22:608–615CrossRefGoogle Scholar
  18. 18.
    Karube I, Matsuoka H, Suzuki S, Watanabe E, Toyama K (1984) Determination of fish freshness with an enzyme sensor system. J Agric Food Chem 32:314–319CrossRefGoogle Scholar
  19. 19.
    Özogul F, Özogul Y (2000) Comparison of methods used for determination of total volatile basic nitrogen (TVB-N) in rainbow trout (Oncorhynchus mykiss). Turk J Zool 24:113–120Google Scholar
  20. 20.
    Ojagh SM, Rezaei M, Razavi SH, Hosseini SMH (2010) Effect of chitosan coatings enriched with cinnamon oil on the quality of refrigerated rainbow trout. Food Chem 120:193–198CrossRefGoogle Scholar
  21. 21.
    Chytiri S, Chouliara I, Savvaidis IN (2004) Microbiological, chemical and sensory assessment of iced whole and filleted aquacultured rainbow trout. Food Microbial 21:157–165CrossRefGoogle Scholar
  22. 22.
    Özyurt G, Kuley E, Özkutuk S, Özogul F (2009) Sensory, microbiological and chemical assessment of the freshness of red mullet (Mullus barbatus) and goldband goatfish (Upeneus moluccensis) during storage in ice. Food Chem 114:505–510CrossRefGoogle Scholar
  23. 23.
    Beatty S (1938) Studies of fish spoilage. II. The origin of trimethylamine produced during the spoilage of cod muscle press juice. J Fish Res Board Canada 4:63CrossRefGoogle Scholar
  24. 24.
    Ruiz-Capillas C, Moral A (2005) Sensory and biochemical aspects of quality of whole bigeye tuna (Thunnus obesus) during bulk storage in controlled atmospheres. Food Chem 89:347–354CrossRefGoogle Scholar
  25. 25.
    Scherer R, Augusti PR, Bochi VC, Steffens C, Fries LLM, Daniel AP, Kubota EH, Neto JR, Emanuelli T (2006) Chemical and microbiological quality of grass carp (Ctenopharyngodon idella) slaughtered by different methods. Food Chem 99:136–142CrossRefGoogle Scholar
  26. 26.
    Goncü Alves AC, Antas SE, Nunes ML (2007) Freshness and quality criteria of iced farmed senegalese sole (Solea senegalensis). J Agric Food Chem 55:3452–3461CrossRefGoogle Scholar
  27. 27.
    Ehira S, Uchiyama H (1974) Freshness lowering rates of cod and seabream viewed from changes in bacterial count, total volatile base and trimethylamine–nitrogen and ATP related compounds. Bull Jpn Soc Sci Fish 40:479–487Google Scholar
  28. 28.
    Chang KLB, Chang J, Shiau CY, Pan BS (1998) Biochemical, microbiological, and densory changes of sea bass (Lateolabrax japonicus) under partial freezing and refrigerated storage. J Agric Food Chem 46:682–686CrossRefGoogle Scholar
  29. 29.
    Bozkurt H, Icier F (2010) Electrical conductivity changes of minced beef–fat blends during ohmic cooking. J Food Eng 96:86–92CrossRefGoogle Scholar
  30. 30.
    Marshall DL, Wiese-Lehigh PL (1997) Comparison of impedance, microbial, sensory, and pH methods to determine shrimp quality. J Aquatic Food Product Technol 6:17–31CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.National Freshwater Fish Processing Technology Research Division Center, College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijingChina
  2. 2.College of ScienceChina Agricultural UniversityBeijingChina

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