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European Food Research and Technology

, Volume 232, Issue 1, pp 83–86 | Cite as

Identification of European commercial cockles (Cerastoderma edule and C. glaucum) by species-specific PCR amplification of the ribosomal DNA ITS region

  • Ruth FreireEmail author
  • Alberto Arias
  • Josefina Méndez
  • Ana Insua
Original Paper

Abstract

We present a novel one-step PCR method for the identification of Cerastoderma edule and C. glaucum. Sequence differences found in the internal transcribed spacer region (ITS) of the ribosomal DNA of the two cockles allowed us to design two species-specific reverse primers. These two primers were used in a multiplex reaction, together with a forward universal primer to amplify specific fragments with different lengths in each cockle (190 and 470 bp in C. edule and C. glaucum, respectively). The successful and specific amplifications obtained for two natural populations in each species as well as in canned products lend support to the usefulness of these markers.

Keywords

C. edule C. glaucum ITS Species-specific PCR 

Notes

Acknowledgments

We thank Dr. Philippe Borsa, Dr. Maciej Wolowicz and Ms. Isabel Pan Souto for supplying samples and Jose García Gil for the technical assistance in the laboratory. This work was funded by the Ministerio de Ciencia y Tecnología and Ministerio de Educación y Ciencia of Spain and Xunta de Galicia through projects IFD97-1295, RZ2006-00005-00-00 and XUGA 10300B97, respectively.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Ruth Freire
    • 1
    Email author
  • Alberto Arias
    • 1
  • Josefina Méndez
    • 1
  • Ana Insua
    • 1
  1. 1.Departamento de Biología Celular y MolecularUniversidade da CoruñaA CoruñaSpain

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