European Food Research and Technology

, Volume 230, Issue 6, pp 915–923 | Cite as

Detection of genetically modified soybean DNA in refined vegetable oils

  • Joana Costa
  • Isabel Mafra
  • Joana S. Amaral
  • M. Beatriz P. P. Oliveira
Original Paper


In this study, four different protocols were tested for their ability to extract DNA from blended refined vegetable oils: the in-house prepared Wizard and CTAB methods and the methods based on the use of the commercial kits Wizard® Magnetic DNA purification system for food and Nucleospin® for food. The performance of the extraction protocols was determined by end-point polymerase chain reaction (PCR) targeting the soybean lectin gene with primers suitable for the amplification of small fragments and confirmed by real-time PCR with specific hydrolysis probes. From the tested protocols, the Nucleospin method was the only one able to produce amplifiable DNA from refined vegetable oils. To verify the presence of Roundup Ready® (RR) soybean, event-specific primers were used for end-point PCR assays. The amplification of trace amounts of RR soybean by real-time PCR confirmed the label statements of two samples. The results highlight the importance of the DNA extraction protocol and the critical choice of PCR primers on processed food matrices, such as refined oils. Considering the few reports and difficulties pointed out in the literature to obtain amplifiable DNA from refined vegetable oils, the present results can be a step forward in the traceability of refined oils regarding authenticity issues and genetically modified organism detection.


Soybean oil Vegetable oil DNA extraction Real-time PCR GMO detection Refined oil 


  1. 1.
    James C (2008) ISAAA Brief 39, accessible in
  2. 2. Accessed on the 14 of January of 2009
  3. 3.
    Mafra I, Ferreira IMPLV, Oliveira MBPP (2008) Eur Food Res Technol 227:649–665CrossRefGoogle Scholar
  4. 4.
    Gryson N, Ronsse F, Messens K, De Loose M, Verleyen T, Dewettinck K (2002) JAOCS 79(2):171–174CrossRefGoogle Scholar
  5. 5.
    Gryson N, Messens K, Dewettinck K (2004) JAOCS 81(3):231–234CrossRefGoogle Scholar
  6. 6.
    Marioda A, Matthäusb B, Eichnerc K, Husseind IH (2006) Eur J Lipid Sci Technol 108:298–308CrossRefGoogle Scholar
  7. 7.
    Breton C, Claux D, Metton I, Skorski G, Bervillé A (2004) J Agric Food Chem 52:531–537CrossRefGoogle Scholar
  8. 8.
    Consolandi C, Palmieri L, Severgnini M, Maestri E, Marmiroli N, Agrimonti C, Baldoni L, Donini P, De Bellis G, Castiglioni G (2008) Eur Food Res Technol 227:1429–1438CrossRefGoogle Scholar
  9. 9.
    Doveri S, Sullivan DMO, Lee D (2006) J Agric Food Chem 54:9221–9226CrossRefGoogle Scholar
  10. 10.
    Martins-Lopes P, Gomes S, Santos E, Guedes-Pinto H (2008) J Agric Food Chem 56:11786–11791CrossRefGoogle Scholar
  11. 11.
    Muzzalupo I, Perri E (2002) Eur Food Res Technol 214:528–531CrossRefGoogle Scholar
  12. 12.
    Muzzalupo I, Pellegrino M, Perri E (2007) Eur Food Res Technol 224:469–475CrossRefGoogle Scholar
  13. 13.
    Hellebrand M, Nagy M, Mörsel JT (1998) Z Lebensm Unters Forsch A 206:237–242CrossRefGoogle Scholar
  14. 14.
    Pauli U, Liniger M, Zimmermann A (1998) Z Lebensm Unters Forsch A 207:264–267CrossRefGoogle Scholar
  15. 15.
    Bogani P, Minunni M, Spiriti MM, Zavaglia M, Tombelli S, Buiatti M, Mascini M (2009) Food Chem 113:658–664CrossRefGoogle Scholar
  16. 16.
    Doveri S, Lee D (2007) J Agric Food Chem 55:4640–4644CrossRefGoogle Scholar
  17. 17.
    Lipp M, Brodmann P, Pietsch K, Pauwels J, Anklam E (1999) J AOAC Int 82:923–928Google Scholar
  18. 18.
    Mafra I, Silva SA, Moreira EJMO, Silva CSF, Oliveira MBPP (2008) Food Control 19:1183–1190CrossRefGoogle Scholar
  19. 19.
    Querci M, Maretti M, Mazzara M (2006) Qualitative detection of MON810 maize, Bt-176 maize and Roundup Ready® soybean by PCR. In Querci M, Jermini M, Eede GV (eds) Training course on The Analysis of Food Samples for the Presence of Genetically Modified Organisms (Session 9). European Commission DG-JRC. Luxembourg: Office for Official Publications of the European Communities (
  20. 20.
    Costa J, Mafra I, Amaral JS, Oliveira MBPP (2010) Food Res Int 43:301–306CrossRefGoogle Scholar
  21. 21.
    ISO 21570 (2005) Foodstuffs–Methods of analysis for the detection of genetically modified organisms and derived products–quantitative nucleic acid based methods, 1st ed. International Standard ISO 21570. ISO, GenevaGoogle Scholar
  22. 22.
    Corbisier P, Broothaerts W, Gioria S, Schimmel H, Burns M, Baoutin AA, Emslie KR, Furui S, Kurosawa Y, Holden MJ, Kim HH, Lee YM, Kawaharasaki M, Sin D, Wang J (2007) J Agric Food Chem 55:3249–3257CrossRefGoogle Scholar
  23. 23.
    Peano C, Lesignoli F, Gulli M, Corradini R, Samson MC, Marchelli R (2005) Anal Biochem 344(2):174–182Google Scholar
  24. 24.
    Pinto AD, Forte V, Guastadisegni MC, Martino C, Schena FP, Tantillo G (2007) Food Control 18:76–80CrossRefGoogle Scholar
  25. 25.
    Pasqualone A, Montemurro C, Summo C, Sabetta W, Caponio F, Blanco A (2007) J Agric Food Chem 55:3857–3862CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Joana Costa
    • 1
  • Isabel Mafra
    • 1
  • Joana S. Amaral
    • 1
    • 2
  • M. Beatriz P. P. Oliveira
    • 1
  1. 1.REQUIMTE, Serviço de Bromatologia, Faculdade de FarmáciaUniversidade do PortoPortoPortugal
  2. 2.ESTiG, Instituto Politécnico de BragançaCampus de Sta.BragançaPortugal

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