Heptaplex real-time PCR for the identification and quantification of DNA from beef, pork, chicken, turkey, horse meat, sheep (mutton) and goat

  • René Köppel
  • Franziska Zimmerli
  • Alda Breitenmoser
Original Paper

Abstract

Meat products are often composed of meat from several species. Due to fraud or incorrect manufacturing processes, different proportions of unexpected or undeclared meat may be incorporated. Pork, beef, chicken, turkey, horse meat, sheep (mutton) and goat are the most common types of meat in these products. To measure the fractional proportion of each of the seven meat types simultaneously, a quantitative multiplex PCR has been developed. This system has proven its applicability in the examination of meat compounds with fractional proportions between 2 and 100%. The uncertainty was 32% or better. In a single analytical step, the multiplex PCR identifies and in certain cases quantifies the most probable species of composed meat products. Additionally, this study illustrates that by using dyes with different wavelength shifts, more targets can be distinguished than channels provided for by the Rotorgene 6000® thermocycler.

Keywords

DNA quantification Multiplex Real-time quantitative PCR Meat Beef Pork Chicken Turkey Horse meat Sheep Goat 

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • René Köppel
    • 1
  • Franziska Zimmerli
    • 1
  • Alda Breitenmoser
    • 1
  1. 1.Official Food Control Authority of the Canton of ZurichZurichSwitzerland

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