European Food Research and Technology

, Volume 229, Issue 5, pp 757–762 | Cite as

Comparative study of microsatellite profiles of DNA from oil and leaves of two Tunisian olive cultivars

  • Rayda Ben Ayed
  • Naziha Grati-Kamoun
  • Fabienne Moreau
  • Ahmed Rebaï
Original Paper

Abstract

The potential of microsatellites in traceability of Tunisian oils was assessed by comparison of the genetic profile for six SSR markers of DNA extracted from oil and leaves of the two Tunisian major olive cultivars: Chemlali and Chetoui. Different protocols of DNA extraction from oil were tested and compared and we found that the most reproducible protocol in terms of successful microsatellites amplification was the Qiagen QIAamp DNA Stool extraction Kit, which we slightly modified. After amplification and automatic genotyping of six SSRs, it has been shown that the pattern of the DNA purified from a monovarietal oil did not correspond exactly to the profile of the same cultivar leaves’ DNA. In fact, we found evidence for the presence in the oil of alleles originating from the pollinators, which are present in the genome of the seed embryo. These alleles appear as minor peaks and could be easily distinguished from the mother tree’s alleles, so they do not interfere with oil traceability.

Keywords

Olive Microsatellites Oil Pollinator Traceability 

Notes

Acknowledgments

This work was supported by the Tunisian Ministry of Agriculture and the ADNid Company (Montpellier, France). We are particularly grateful to Jean-Fraçois Ballester, director of ADNid and to all its staff.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Rayda Ben Ayed
    • 1
    • 2
  • Naziha Grati-Kamoun
    • 2
  • Fabienne Moreau
    • 3
  • Ahmed Rebaï
    • 1
  1. 1.Centre of Biotechnology of SfaxSfaxTunisia
  2. 2.Olive Tree InstituteSfaxTunisia
  3. 3.Société ADNid, SARL ADNidMontpellierFrance

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