Isolation of an anti-tumor polysaccharide from Auricularia polytricha (jew’s ear) and its effects on macrophage activation
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To investigate the active compound and its mechanism of the anti-tumor effects of Auricularia polytricha, the polysaccharides were isolated and its effects on macrophages were tested. By alcohol-precipitation, DEAE-cellulose ion exchange, gel permeation chromatography and in vivo anti-tumor test on the sarcoma 180 tumor-bearing mice, a fraction of polysaccharides (namely APPIIA) with significant anti-tumor effect was isolated. APPIIA can enhance the neutral red phagocytosis of macrophages. It can also stimulate the nitric oxide (NO) release and cytokines secretion of macrophage in a dose-dependent manner. By quantitative RT-PCR method, it can be found that APPIIA can augment the mRNA transcription of inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6. The results of western blotting showed that it can also increased the protein expression of iNOS. These results suggest that APPIIA is one of effective anti-tumor agents and its anti-tumor effect may exhibit via the macrophage activation.
KeywordsAuricularia polytricha Polysaccharides Anti-tumor Macrophage activation Cytokine NO
We would like to send our special thanks to Expert Committee of Edible Fungi of Deyang for providing Auricularia polytricha and to Ms Huang for her earnest animal care.
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