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Isolation of an anti-tumor polysaccharide from Auricularia polytricha (jew’s ear) and its effects on macrophage activation

  • Mengyao Yu
  • Xiaoyan Xu
  • Yuan Qing
  • Xia Luo
  • Zhirong Yang
  • Linyong ZhengEmail author
Original Paper

Abstract

To investigate the active compound and its mechanism of the anti-tumor effects of Auricularia polytricha, the polysaccharides were isolated and its effects on macrophages were tested. By alcohol-precipitation, DEAE-cellulose ion exchange, gel permeation chromatography and in vivo anti-tumor test on the sarcoma 180 tumor-bearing mice, a fraction of polysaccharides (namely APPIIA) with significant anti-tumor effect was isolated. APPIIA can enhance the neutral red phagocytosis of macrophages. It can also stimulate the nitric oxide (NO) release and cytokines secretion of macrophage in a dose-dependent manner. By quantitative RT-PCR method, it can be found that APPIIA can augment the mRNA transcription of inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6. The results of western blotting showed that it can also increased the protein expression of iNOS. These results suggest that APPIIA is one of effective anti-tumor agents and its anti-tumor effect may exhibit via the macrophage activation.

Keywords

Auricularia polytricha Polysaccharides Anti-tumor Macrophage activation Cytokine NO 

Notes

Acknowledgments

We would like to send our special thanks to Expert Committee of Edible Fungi of Deyang for providing Auricularia polytricha and to Ms Huang for her earnest animal care.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Mengyao Yu
    • 1
    • 3
  • Xiaoyan Xu
    • 3
  • Yuan Qing
    • 1
    • 3
  • Xia Luo
    • 3
  • Zhirong Yang
    • 1
  • Linyong Zheng
    • 1
    • 2
    Email author
  1. 1.College of Life ScienceSichuan UniversityChengduChina
  2. 2.Soil and Fertilizer InstituteSichuan Academy of Agricultural SciencesChengduChina
  3. 3.Sichuan Institute of Chinese Materia MedicaChengduChina

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