European Food Research and Technology

, Volume 227, Issue 2, pp 425–431 | Cite as

Antioxidant capacity of walnut (Juglans regia L.): contribution of oil and defatted matter

  • Sara Arranz
  • Jara Pérez-Jiménez
  • Fulgencio Saura-Calixto
Original Paper

Abstract

Several studies have concluded that walnut exhibits greater antioxidant capacity than any other nuts. However, the contribution to antioxidant capacity of the two major fractions of walnut (defatted matter and oil) is unknown, and the aim of the present work is to elucidate it. Antioxidant capacity was evaluated in walnut oil, defatted matter, and whole walnut. The results showed that the defatted matter provided the bulk of the antioxidant capacity (estimated about 332 μmol Trolox/g dm) of this nut, a major proportion derived from insoluble tannins. The contribution of walnut oil to the overall antioxidant capacity of walnut (FRAP and ABTS assays) is less than a 5%. It was observed that oil interfered in the determination of antioxidant capacity of whole walnut, a fact that could affect the data reported in the literature. Separate determination of oil and defatted mater antioxidant capacity is recommended.

Keywords

Defatted matter Antioxidants Walnut Oil Juglans regia L. 

Abbreviations

ABTS

2,2′-Azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid)

AE

Antiradical efficiency

CUPRAC

Cupric reducing/antioxidant capacity

DPPH

2,2 Diphenyl-1-picrylhydrazyl

FRAP

Ferric reducing/antioxidant power

LDLs

Low-density lipoproteins

ORAC

Oxygen radical absorbance capacity

TBARs

Thiobarbituric acid reactive substances

TRAP

Total peroxyl radical trapping antioxidant activity

Notes

Acknowledgments

The present research was performed under the financial support of the Spanish Ministry of Education and Science (project AGL 2004-07579-C04-01/ALI). J. Pérez-Jiménez thanks the Consejo Superior de Investigaciones Científicas for granting her an I3P scholarship, financed by the European Social Fund. S. Arranz thanks the Ministerio de Educación y Ciencia for granting her an FPI scholarship.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Sara Arranz
    • 1
  • Jara Pérez-Jiménez
    • 1
  • Fulgencio Saura-Calixto
    • 1
  1. 1.Department of Metabolism and NutritionInstituto del Frío, CSICMadridSpain

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