European Food Research and Technology

, Volume 226, Issue 6, pp 1513–1524 | Cite as

Modelling the limit of detection in real-time quantitative PCR

Original Paper

Abstract

The limit of detection (LOD) is a critical performance characteristic of an assay that requires careful evaluation during method validation. However, formal calculations for the LOD do not take into account atypical data sets that are generated from real-time PCR techniques, which can be non-normally distributed, truncated, and heteroscedastic. Experimental data sets for the quantification of genetically modified (GM) material were produced using real-time PCR, in order to model the LOD. A bootstrapping computer simulation calculated the probabilities of detecting PCR positive test results from these data sets, and computer modelling defined a function from the resulting probability plots. The LOD was modelled as a function of sample replication level and cycle threshold values. The broad applicability of this bootstrapping and data modelling approach should be of general interest to laboratories conducting trace-level detection.

Keywords

Limit of detection (LOD) Real-time quantitative PCR Trace detection Sensitivity 

Notes

Acknowledgments

The work presented here was part of the “Government Chemist 2005–2008 Programme” and was funded by the Department of Trade and Industry, UK.

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Copyright information

© LGC Limited 2007

Authors and Affiliations

  1. 1.Bio-Molecular InnovationLGC LimitedTeddingtonUK

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