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European Food Research and Technology

, Volume 226, Issue 6, pp 1429–1437 | Cite as

Comparison of glucosinolate levels in commercial broccoli and red cabbage from conventional and ecological farming

  • Michael Meyer
  • Sieghard T. AdamEmail author
Original Paper

Abstract

Broccoli heads and red cabbage of both conventional and ecological origin were purchased from the market at monthly intervals over a 1-year period. After freeze-drying of the samples the glucosinolates were extracted, enzymatically desulphated and analyzed by HPLC-UV. Glucoraphanin, glucobrassicin and neo-glucobrassicin turned out to be the predominant glucosinolates in broccoli. Red cabbage contained similar amounts of glucoraphanin and glucobrassicin but, in addition, appreciable amounts of glucoiberin, progoitrin, sinigrin, gluconapin and glucoerucin, while neo-glucobrassicin occurred at trace levels only. No significance was found comparing the contents of glucoraphanin in the two cultivation groups for either broccoli or red cabbage. Organic broccoli and red cabbage both contained significantly higher amounts of glucobrassicin than their conventionally grown counterparts. Conventional crops of red cabbage yielded significantly higher quantities of gluconapin than ecological crops. Broccoli imported from Spain and Italy during the winter months yielded levels of glucosinolates similar to those of the home-grown products available in summer and autumn.

Keywords

Broccoli Red cabbage Profiles of glucosinolates HPLC-UV Conventional and ecological agriculture 

Notes

Acknowledgments

The authors wish to thank Dipl.-Inform. L. Korn who carried out the statistical data analysis, Dipl.-Ing. A. Rathjen for his assistance with graphs and for helpful discussions, and Ms. D. Inkster for proofreading the manuscript.

References

  1. 1.
    Verhoeven DTH, Goldbohm RA, van Poppel G, Verhagen H, van den Brandt PA (1996) Epidemiological studies on brassica vegetables and cancer risk. Cancer Epidemiol Biomarkers Prev 5:733–748Google Scholar
  2. 2.
    Giuvannucci E, Rimm EB, Liu Y, Stampfer MJ, Willett WC (2003) A prospective study of cruciferous vegetables and prostate cancer. Cancer Epidemiol Biomarkers Prev 12:1403–1409Google Scholar
  3. 3.
    Voorips LE, Goldbohm RA, Verhoeven DTH, van Poppel GAFC, Sturmans F, Hermus RJJ, van den Brandt PA (2000) Vegetable and fruit consumption and lung cancer risk in the Netherlands cohort study on diet and cancer. Cancer Causes Control 11:101–115CrossRefGoogle Scholar
  4. 4.
    Ambrosone CB, McCann SE, Freudenheim JL, Marshall JR, Zhang Y, Shields PG (2004) Breast cancer risk in premenopausal women is inversely associated with consumption of broccoli, a source of isothiocyanates, but is not modified by GST genotype. J Nutr 134:1134–1138Google Scholar
  5. 5.
    Hara M, Hanaoka T, Kobayashi M, Otani T, Adachi HY, Montani A, Natsukawa S, Shaura K, Koizumi Y, Kasuga Y, Matsuzawa T, Ikekawa T, Sasaki S, Tsugane S (2003) Cruciferous vegetables, mushrooms, and gastrointestinal cancer risks in a multicenter, hospital-based case-control study in Japan. Nutr Cancer 46:138–147CrossRefGoogle Scholar
  6. 6.
    Michaud DS, Spiegelman D, Clinton SK, Rimm EB, Willet WC, Giovannuci EL (1999) Fruit and vegetable intake and incidence of bladder cancer in a male prospective cohort. J Natl Cancer Inst 91:605–613CrossRefGoogle Scholar
  7. 7.
    Joshipura KJ, Ascherio A, Manson J, Stampfer MJ, Rimm EB, Speizer FE, Hennekens CH, Spiegelman D, Willett WC (1999) Fruit and vegetable intake in relation to risk of ischemic stroke. JAMA 282:1233–1239CrossRefGoogle Scholar
  8. 8.
    Wu L, Ashraf N, Facci M, Wang R, Paterson PG, Ferrie A, Juurlink BH (2004) Dietary approach to attenuate oxidative stress, hypertension, and inflammation in the cardiovascular system. Proc Natl Acad Sci USA 101:7094–7099CrossRefGoogle Scholar
  9. 9.
    Martínez A, Cambero I, Ikken Y, Marín ML, Haza AI, Morales P (1998) Protective effect of broccoli, onion, carrot, and licorice extracts against cytotoxicity of N-nitrosamines evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. J Agric Food Chem 46:585–589. doi: 101021/jf970636i CrossRefGoogle Scholar
  10. 10.
    Brandi G, Schiavano GF, Zaffaroni N, De Marco C, Paiardini M, Cervasi B, Magnani M (2005) Mechanisms of action and antiproliferative properties of Brassica oleracea juice in human breast cancer cell lines. J Nutr 135:1503–1509Google Scholar
  11. 11.
    Kassie F, Uhl M, Rabot S, Grasl-Kraupp B, Verjerk R, Kundi M, Chabicovsky M, Schulte-Hermann R, Knasmüller S (2003) Chemoprevention of 2-amino-3-methylimidazo (4, 5-f) quinoline (IQ)-induced colonic and hepatic preneoplastic lesions in the F344 rat by cruciferous vegetables administered simultaneously with the carcinogen. Carcinogenesis 24:255–261. doi: 10.1093/carcin/24.2.255 CrossRefGoogle Scholar
  12. 12.
    Bones AM, Rossiter J (1996) The myrosinase-glucosinolate system, its organisation and biochemistry. Physiol Plant 97:194–208CrossRefGoogle Scholar
  13. 13.
    Zhang Y, Talalay P, Cho C-G, Posner GH (1992) A major inducer of anticarcinogenic protective enzymes from broccoli: isolation and elucidation of structure. Proc Natl Acad Sci USA 89:2399–2403CrossRefGoogle Scholar
  14. 14.
    Barceló S, Mace K, Pfeifer AMA, Chipman JK (1998) Production of DNA strand breaks by N-nitrosodimethylamine and 2-amino-3-methylimidazo[4,5-f] quinoline in THLE cells expressing human CYP isoenzymes and inhibition by sulforaphane. Mutat Res 402:111–120Google Scholar
  15. 15.
    Brandt K, Molgaard JPM (2001) Organic agriculture: does it enhance or reduce the nutritional value of food plants. J Sci Food Agric 81:924–931. doi: 10.1002/jsfa.903 CrossRefGoogle Scholar
  16. 16.
    Mäder P, Fliessbach A, Dubois D, Gunst L, Fried P, Niggli U (2002) Soil fertility and biodiversity in organic farming. Science 296:1694–1697CrossRefGoogle Scholar
  17. 17.
    Woese K, Lange D, Boess C, Bögl KW (1997) A comparison of organically and conventionally grown foods—results of a review of the relevant literature. J Sci Food Agric 74:281–293CrossRefGoogle Scholar
  18. 18.
    EC (1990) Oil seeds-determination of glucosinolates, high performance liquid chromatography. Official J Eur Comm L170:28–34Google Scholar
  19. 19.
    Chiang CK, Pusateri DJ, Leitz REA (1998) Gas chromatography/mass spectrometry method for the determination of sulforaphane and sulforaphane nitrile in broccoli. J Agric Food Chem 46:1018–1021. doi: 10.1021/jf970572b CrossRefGoogle Scholar
  20. 20.
    Matthaeus B, Luftmann H (2000) Glucosinolates in members of the family Brassicaceae: separation and identification by LC/ESI-MS-MS. J Agric Food Chem 48:2234–2239. doi: 10.1021/jf991306w CrossRefGoogle Scholar
  21. 21.
    Artés F, Vallejo F, Martínez JA (2001) Quality of broccoli as influenced by film wrapping during shipment. Eur Food Res Technol 213:480–483. doi: 10.1007/s002170100390 CrossRefGoogle Scholar
  22. 22.
    Rosa EAS, Rodrigues AS (2001) Total and individual glucosinolate content in 11 broccoli cultivars grown in early and late seasons. Hort Sci 36:56–59Google Scholar
  23. 23.
    Hansen M, Moller P, Sorensen H (1995) Glucosinolates in broccoli stored under controlled atmosphere. J Am Soc Hort Sci 120:1069–1074Google Scholar
  24. 24.
    Schütze W, Mandel F, Schulz H (1999) Identifizierung von Glucosinolaten in Rettich (Raphanus sativus L) und Kreuzungen aus R. sativus L. × Brassica oleracea L. (Raphanobrassica) mittels LC–MS. Nahrung 43:245–248CrossRefGoogle Scholar
  25. 25.
    Vallejo F, Tomás-Barberán FA, Gonzales Benavente-García A, García-Viguera C (2003) Total and individual glucosinolate contents in inflorescences of eight broccoli cultivars grown under various climatic and fertilisation conditions. J Sci Food Agric 83:307–313. doi: 10.1002/jsfa.1320 CrossRefGoogle Scholar
  26. 26.
    Charron CS, Saxton AM, Sams CE (2005) Relationship of climate and genotype to seasonal variation in the glucosinolate-myrosinase system. I. Glucosinolate content in ten cultivars of Brassica oleracea grown in fall and spring seasons. J Sci Food Agric 85:671–681. doi: 10.1002/jsfa.1880 CrossRefGoogle Scholar
  27. 27.
    Vallejo F, Tomás-Barberán FA, García-Viguera C (2002) Potential bioactive compounds in health promotion from broccoli cultivars grown in Spain. J Sci Food Agric 82:1293–1297. doi: 10.1002/jsfa.1183 CrossRefGoogle Scholar
  28. 28.
    Rodrigues AS, Rosa EAS (1999) Effect of post-harvest treatments on the level of glucosinolates in broccoli. J Sci Food Agric 79:1028–1032CrossRefGoogle Scholar
  29. 29.
    Vallejo F, Tomás-Barberán F, García-Viguera C (2003) Health-promoting compounds in broccoli as influenced by refrigerated transport and retail sale period. J Agric Food Chem 51:3029–3034. doi: 10.1021/jf021065j CrossRefGoogle Scholar
  30. 30.
    Rangkadilok N, Tomkins B, Nicolas ME, Premier RR, Bennet RN, Eagling DR, Taylor PWJ (2002) The effect of post-harvest and packaging treatments on glucoraphanin concentration in broccoli (Brassica oleracea var. italica). J Agric Food Chem 50:7386–7391. doi: 10.1021/jf0203592 CrossRefGoogle Scholar
  31. 31.
    Kushad M, Brown AF, Kurilich AC, Juvik JA, Klein BP, Wallig MA, Jeffery EH (1999) Variation of glucosinolates in vegetable crops of Brassica oleracea. J Agric Food Chem 47:1541–1548. doi: 10.1021/jf980985s CrossRefGoogle Scholar
  32. 32.
    Uhl M, Kassie F, Rabot S, Grasl-Kraupp B, Chakraborty A, Laky B Kundi B, Knasmüller S (2004) Effect of common brassica vegetables (Brussels sprout and red cabbage) on the development of preneoplastic lesions induced by 2-amino-3-methylimidaz [4, 5-f]quinoline(IQ) in liver and colon of Fischer 344 rats. J Chromatography B 802:225–230. doi: 10.1016/j.jchromb.2003.11.014 CrossRefGoogle Scholar
  33. 33.
    Ciska E, Martyniak-Przybyszewska B, Kozlowska H (2000) Content of glucosinolates in cruciferous vegetables grown at the same site for two years under different climatic conditions. J Agric Food Chem 48:2862–2867. doi: 10.1021/jf981373a CrossRefGoogle Scholar
  34. 34.
    Chong C, Berard LC (1983) Changes in glucosinolates during refrigerated storage of cabbage. J Am Soc Hort Sci 108:688–691Google Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Institute of Chemistry and BiologyFederal Research Centre for Nutrition and FoodKarlsruheGermany

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