European Food Research and Technology

, Volume 226, Issue 5, pp 1047–1055 | Cite as

Bio-preservation of fresh-cut tomatoes using natural antimicrobials

  • J. Fernando Ayala-Zavala
  • Gemma Oms-Oliu
  • Isabel Odriozola-Serrano
  • Gustavo A. González-Aguilar
  • Emilio Álvarez-Parrilla
  • Olga Martín-BellosoEmail author
Original Paper


Effects of several natural volatile compounds such as methyl jasmonate (MJ), ethanol (ETOH), their combination (MJ–ETOH), tea tree oil (TTO) and garlic oil (GO) were evaluated on fresh-cut tomato storage at 5 °C for 15 days. Microbial growth, in-packaged atmosphere, quality attributes (firmness and color) and bioactive compounds (lycopene content, ascorbic acid and phenolic compounds) of fresh-cut tomatoes were determined. The shelf-life based on microbial growth was longer on fresh-cut tomatoes treated with natural volatile compounds than in control fresh-cut tomatoes. Treatment with ETOH combined with MJ was more effective in suppressing microbial proliferation than the individual treatments with each natural volatile compound. The lowest in-packaged atmosphere modification was detected in fresh-cut tomatoes treated by ETOH. In addition, this treatment better maintained firmness and color attributes than those treated with the other antimicrobial compounds. Related to the bioactive compounds, the use of MJ let keep higher content of lycopene, ascorbic acid and phenolic compounds than the other treatments. Thus, the shelf-life of fresh-cut tomatoes could be prolonged by the use of natural antimicrobials, which do not have significant negative effects on health-related compounds.


Natural antimicrobials Fresh-cut tomato In-packaged atmosphere Shelf-life Bioactive compounds 



We are indebted to Alejandra Rojas Graü for her skillful technical assistance.This research was supported by The Departament d’Universitats Recerca i Societat de la Informació (DURSI) of the Generalitat de Catalunya (Spain) and The Mexican National Council of Science and Technology (CONACYT), Centro de Investigación en Alimentación y Desarrollo A.C. (CIAD, A.C.). This work is included in the project XI.22 “Desarrollo de Tecnologías para la Conservación de Productos Vegetales Frescos Cortados” financed by CYTED (Spain).


  1. 1.
    Watada AE, Abe K, Yamuchi N (1990) Food Technol 44(5):116–118, 120–122Google Scholar
  2. 2.
    Varoquaux P, Wiley R (1994) Biological and biochemical changes in minimally processed refrigerated fruits and vegetables. In: Wiley (ed) Minimally processed refrigerated fruits and vegetables. Chapman-Hall, New York, pp 226–268Google Scholar
  3. 3.
    Cantwell M (1998) Introduction to fresh-cut products: maintaining quality and safety. University of California, Postharvest Hort., Series No. 10, Section 1Google Scholar
  4. 4.
    Gross KC, Saltveit ME (1982) J Am Soc Hort Sci 107:328–330Google Scholar
  5. 5.
    Edwards J, Saltveit ME, Henderson WR (1983) J Am Soc Hort Sci 108:512–514Google Scholar
  6. 6.
    Mencarelli F, Saltveit ME (1988) J Am Soc Hort 113:742–745Google Scholar
  7. 7.
    Mencarelli F, Savarese P, Saltveit ME (1991) HortSci 26:566–569Google Scholar
  8. 8.
    Artés F, Conesa MA, Hernández S, Gil MI (1999) Postharv Biol Technol 17:153–162CrossRefGoogle Scholar
  9. 9.
    Toivonen PMA, DeEll JR (2002) Physiology of fresh-cut fruits and vegetables. In: Lamikanra (ed) Fresh-cut fruits and vegetables: science, technology and market, CRC Press, Boca Raton, pp 91–123Google Scholar
  10. 10.
    Buta IT, Moline HE (1998) J Agric Food Chem 46:1253–1256CrossRefGoogle Scholar
  11. 11.
    Droby S, Porat R, Cohen L, Weiss B, Shapiro B, Philosoph-Hadas S, Meir S (1999) J Am Soc Hort Sci 124:184–188Google Scholar
  12. 12.
    Ayala-Zavala JF, Wang SY, Wang CY, Gonzalez-Aguilar GA (2005) Eur Food Res Technol 221(6):731–738CrossRefGoogle Scholar
  13. 13.
    Lichte A, Zutkhy Y, Sonego L (2002) Postharv Biol Technol 24:301–308CrossRefGoogle Scholar
  14. 14.
    Saltveit ME, Sharaf AR (1992) J Am Soc Hort Sci 117:793–798Google Scholar
  15. 15.
    Naganawa R, Iwata N, Ishikawa K, Fukyda H, Fujino T, Suzuki A (1996) Appl Environ Microbiol 62:4238–4242Google Scholar
  16. 16.
    Benkeblia N (2004) Lebensm Wiss Technol 37(2):263–268CrossRefGoogle Scholar
  17. 17.
    Giovanelli G, Lavelli V, Peri C, Nobili S (1999) J Sci Food Agric 79:1583–1588CrossRefGoogle Scholar
  18. 18.
    Gahler S, Otto K, Bohm V (2003) J Agric Food Chem 51:7962–7968CrossRefGoogle Scholar
  19. 19.
    Horwitz W (2000) Official methods of analysis, 17th edn. Association of Official Analytical Chemists (AOAC), Washington, DCGoogle Scholar
  20. 20.
    ISO (1991) Methods for microbiological examination of food and animal feeding stuffs, ISO, GenevaGoogle Scholar
  21. 21.
    ISO (1988) General guidance for enumeration of yeasts and moulds. ISO, GenevaGoogle Scholar
  22. 22.
    American Public Health Association (1992) Compendium of methods for the microbiological examination of foods, 3rd edn. APHA, Washington, DCGoogle Scholar
  23. 23.
    Davis AR, Fish WW, Perkins-Veazie P (2003) Postharv Biol Technol 28:425–430CrossRefGoogle Scholar
  24. 24.
    Fish WW, Perkins-Veazie P, Collins JK (2002) J Food Compos Anal 15(3):309–317CrossRefGoogle Scholar
  25. 25.
    Singleton VL, Orthofer RM, Lamuela-Raventos RM (1999) Meth Enzymol 299:152–178CrossRefGoogle Scholar
  26. 26.
    Carlin F, Nguyen-the C, Chambroy Y (1990) Int J Food Sci Technol 25:110–119Google Scholar
  27. 27.
    Carson CF, Riley TV (1995) J Appl Bacteriol 78:264–269Google Scholar
  28. 28.
    Karalabut OA, Arslan U, Kuruoglu G, Ozgenc T (2004) J Phytopathol 152:298–303CrossRefGoogle Scholar
  29. 29.
    Litcher A, Zutkhy Y, Sonego L, Dvir O, Kaplunov T, Sarig P, BenArie R (2002) Postharvest Biol Technol 24:301–308CrossRefGoogle Scholar
  30. 30.
    Cox SD, Mann CM, Markham JL, Bell HC, Gustafson JE, Warmington JR, Wyllie SG (2000) J Appl Microbiol 88:170–175CrossRefGoogle Scholar
  31. 31.
    Farmer EE (1994) Plant Mol Biol 26:1423–1437CrossRefGoogle Scholar
  32. 32.
    Han JH, Tao WY, Hao HK (2006) J Food Sci 71(5):S385–S389CrossRefGoogle Scholar
  33. 33.
    Perez AG, Sanz C, Olias R, Olias JM (1997) J Agric Food Chem 24:3733–3737CrossRefGoogle Scholar
  34. 34.
    Saniewsky M (1997) The role of jasmonates in ethylene biosynthesis. In: Kanellis (ed) Biology and biotechnology of the plant hormone ethylene, Kluwer, Dordrecht, pp 39–45Google Scholar
  35. 35.
    Saltveit ME, Mencarelli F (1988) J Am Soc Hort Sci 113:572–576Google Scholar
  36. 36.
    Soliva-Fortuny RC, Oms-Oliu G, Martín-Belloso O (2002) J Food Sci 67:1958–1963CrossRefGoogle Scholar
  37. 37.
    Yanuriati A, Geoffrey PS, Richard NR (1999) J Sci Food Agric 79:995–1002CrossRefGoogle Scholar
  38. 38.
    González-Aguilar GA, Buta JG, Wang CY (2001) J Sci Food Agric 81:1244–1249CrossRefGoogle Scholar
  39. 39.
    Fan X, Mattheis JP, Fellman JK, Patterson ME (1997) J Agric Chem 45:208–211CrossRefGoogle Scholar
  40. 40.
    Britton G, Hornero-Méndez D (2004). Carotenoids and colour in fruit and vegetables. In: Britton, Liaaen-Jensen, Pfander (eds) Carotenoids handbook, (Compiledby AZ Mercadante and ES Egeland), Birkhauser Verlag, Basel, pp 11–27Google Scholar
  41. 41.
    Wolucka BA, Goossens A, Inzé D (2005) J Exp Botany 419:2527–2538CrossRefGoogle Scholar
  42. 42.
    Chanjirakul K, Wang SY, Wang CY, Siriphanich J (2006) Posthar Biol Technol 40:106–115CrossRefGoogle Scholar
  43. 43.
    Liptay A (1984) Acta Hortic 163:271–276, 547Google Scholar
  44. 44.
    Franceschi VR, Tarlyn NM (2002) Plant Physiol 130(2):649–656CrossRefGoogle Scholar
  45. 45.
    González-Aguilar GA, Tiznado-Hernández ME, Zavaleta-Gatica R, Martínez-Téllez MA (2004) Biochem Biophys Res Commun 313:704–711Google Scholar
  46. 46.
    Gross D, Parthier B (1994) J Plant Growth Regulation 13: 93–114CrossRefGoogle Scholar
  47. 47.
    Gundlach H, Müller M.J, Kutchan TM, Zenk MH (1992) Proc Natl Acad Sci 89:2389–93CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • J. Fernando Ayala-Zavala
    • 1
  • Gemma Oms-Oliu
    • 2
  • Isabel Odriozola-Serrano
    • 2
  • Gustavo A. González-Aguilar
    • 1
  • Emilio Álvarez-Parrilla
    • 3
  • Olga Martín-Belloso
    • 2
    Email author
  1. 1.Centro de Investigación en Alimentación y Desarrollo, AC (CIAD, AC)HermosilloMexico
  2. 2.Department of Food Technology, UTPV-CeRTAUniversity of LleidaLleidaSpain
  3. 3.Departamento de Ciencias BásicasUniversidad Autónoma de Ciudad JuárezCiudad JuárezMexico

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