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European Food Research and Technology

, Volume 226, Issue 3, pp 507–515 | Cite as

Antioxidant activity of Dioscorea batatas Decne glycoprotein

  • Phil-Sun Oh
  • Kye-Taek Lim
Original Paper

Abstract

Dioscorea batatas Decne (DBD) has been traditionally used as folk medicine and health food in Korea. One glycoprotein was isolated from DBD and confirmed to have 30 kDa molecular weight. The DBD glycoprotein was tested its antioxidative activity and characterized in various chemical conditions. The DBD glycoprotein has the optimal free radical scavenging activities in acidic and neutral pH and up to 85 °C. In the M2+ ions (Ca2+, Mn2+, and Mg2+) in the presence of EDTA, the activities of DBD glycoprotein reduced, compared to DBD glycoprotein alone without metal ion. Interestingly, the results in this study indicated that the activities of DBD glycoprotein do not depend on the presence of EDTA. Interestingly, when DBD glycoprotein was treated with deactivation agents (pronase E or NaIO4), scavenging activity of DBD glycoprotein was decreased. The anti-oxidative effects of DBD glycoprotein on hydroxyl radicals in cell-free system revealed, and the DBD glycoprotein has remarkable scavenging effects on hydroxyl radicals generated by G/GO. Furthermore, the results in this study showed that the DBD glycoprotein (200 μg/ml) significantly inhibits intracellular ROS amounts and protects from cytotoxicity in primary mouse splenocyte culture treated with GO (30 mU/ml). Therefore, we speculate that DBD glycoprotein has an antioxidative potential as one of natural antioxidants.

Keywords

Dioscorea batatas Decne (DBD) glycoprotein Anti-oxidative activity Intracellular reactive oxygen species 

Notes

Acknowledgments

This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF-2006-311-F00108).

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Molecular Biochemistry Laboratory, Biotechnology Research InstituteChonnam National UniversityGwangju CitySouth Korea
  2. 2.Center for the Control of Animal Hazards Using Biotechnology (BK21)Chonnam National UniversityGwangju CitySouth Korea

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