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European Food Research and Technology

, Volume 226, Issue 1–2, pp 215–223 | Cite as

Evidence of mixed wild populations of Oenococcus oeni strains during wine spontaneous malolactic fermentations

  • Isabel López
  • Carmen Tenorio
  • Myriam Zarazaga
  • Marta Dizy
  • Carmen Torres
  • Fernanda Ruiz-LarreaEmail author
Original Paper

Abstract

Two hundred and four bacterial isolates from Rioja red wines undergoing spontaneous malolactic fermentation (MLF) were studied. Bacterial species was determined both by microbiological identification methods and by specific PCR analysis. Oenococcus oeni was shown to be the predominant species (98.5% of total isolates). Pulsed field gel electrophoresis (PFGE) of chromosomal DNA digested with SfiI was used to differentiate individual strains of O. oeni. A wide variety of restriction digest patterns were detected, which indicated a rich biodiversity of indigenous strains. Most fermentations (37 out of 41) showed from 2 to 6 clones growing in the same tank. Five O. oeni strains were the most frequently found, appearing in more than three of the 13 studied wineries, and most times in combination with other less frequently found strains. PFGE was shown to be a suitable method for strain differentiation, for monitoring individual strains and determining which strains actually survive and carry out MLF. A high genotypic heterogeneity of wild O. oeni strains was demonstrated and 90% of the studied wines showed mixed populations of O. oeni strains during MLF.

Keywords

Oenococcus oeni Wine PFGE Spontaneous malolactic fermentation 

Notes

Acknowledgements

This work was supported by FEDER-CYCIT grant 2FD97-1475 of the European Community and the Spanish Ministry of Science and Technology, by the I.N.I.A. grant VIN00-043-C3, by the University of La Rioja grants API00/B30 and API01/B34, and by the Autonomous Community of La Rioja grant ACPI-ANGI/2000-04. I. López was supported by doctorate grants of the Spanish Ministry of Education and Culture (grant no. AP99 16580683).

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Isabel López
    • 1
  • Carmen Tenorio
    • 1
  • Myriam Zarazaga
    • 1
  • Marta Dizy
    • 1
  • Carmen Torres
    • 1
  • Fernanda Ruiz-Larrea
    • 1
    Email author
  1. 1.Department of Food and AgricultureUniversity of La RiojaLogroñoSpain

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