European Food Research and Technology

, Volume 226, Issue 1–2, pp 71–79 | Cite as

Antioxidant activities of phlorotannins purified from Ecklonia cava on free radical scavenging using ESR and H2O2-mediated DNA damage

  • Gin-Nae Ahn
  • Kil-Nam Kim
  • Seon-Heui Cha
  • Choon-Bok Song
  • Jehee Lee
  • Moon-Soo Heo
  • In-Kyu Yeo
  • Nam-Ho Lee
  • Young-Heun Jee
  • Jin-Soo Kim
  • Min-Soo Heu
  • You-Jin Jeon
Original Paper

Abstract

The potential antioxidant activities of three phlorotannins (phloroglucinol, eckol and dieckol) purified from Ecklonia cava collected in Jeju Island were investigated to evaluate their potential value as the natural products for foods or cosmetic application. In this study, antioxidant activities were measured by electron spin resonance spectrometry (ESR) technique for scavenging effects of free radicals such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), alkyl, hydroxyl (HO) and superoxide anion radical (O2•−) and by comet assay for protecting effects against H2O2-mediated DNA damage. The results show that all the phlorotannins have the potential DPPH, alkyl, hydroxyl and superoxide radical scavenging activities. Especially, eckol samples scavenged around 93% of DPPH at 0.25, 0.5, 1 mg/mL of concentrations and were higher than the other phlorotannins, such as phloroglucinol and dieckol samples. Also, protecting effects of the phlorotannins against H2O2-mediated DNA damage increased with increased concentrations of the samples in the L5178 mouse T-cell lymphoma cell lines (L5178Y-R). In conclusion, these results suggest that the three phlorotannins purified from E. cava have the potential inhibitory effect on H2O2-mediated DNA damage and harmful free radicals and can be used as antioxidants in cosmetic, foods and drug industry.

Keywords

Antioxidant activity Ecklonia cava Phlorotannin Electron spin resonance (ESR) Comet assay DNA damage 

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Gin-Nae Ahn
    • 1
  • Kil-Nam Kim
    • 1
  • Seon-Heui Cha
    • 1
  • Choon-Bok Song
    • 1
  • Jehee Lee
    • 1
  • Moon-Soo Heo
    • 1
  • In-Kyu Yeo
    • 1
  • Nam-Ho Lee
    • 2
  • Young-Heun Jee
    • 3
  • Jin-Soo Kim
    • 4
  • Min-Soo Heu
    • 4
  • You-Jin Jeon
    • 1
    • 5
  1. 1.Faculty of Applied Marine Science, Cheju National UniversityJejuKorea
  2. 2.Department of Chemistry, Cheju National UniversityJejuKorea
  3. 3.Department of Veterinary Medicine, Applied Radiological Science Institute, Cheju National UniversityJejuKorea
  4. 4.Division of Marine Bioscience, Gyeongsang National UniversitySeoulKorea
  5. 5.Applied Radiological Science Research Institute, Cheju National UniversityJejuKorea

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