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Detection of genetically modified rice: a construct-specific real-time PCR method based on DNA sequences from transgenic Bt rice

  • Dietrich Mäde
  • Christine Degner
  • Lutz Grohmann
Original Paper

Abstract

Genetically modified rice varieties developed in China are close to approval for agricultural cultivation and production. However, so far no method has been reported for specific detection of transgenic varieties of this crop. In the present study, rice seeds assumed to consist of field-tested Bt rice (‘Anti-pest Shanyou 63’ and ‘Anti-pest Jinyou 63’) were used as reference material to determine transgenic DNA sequences. The transition between the cryIA(b) and cryIA(c) fusion gene and the nopaline synthase terminator (nos) sequence was used to develop a construct-specific real-time PCR based detection method. This Bt rice specific detection system was combined with a recently published quantitative real-time PCR method for the rice-specific (Oryza sativa L.) reference gene gos9. The complete PCR assay for detection of transgenic Bt rice was in-house validated and the limit of quantification was found to be below 0.1% Bt rice relative to the rice content. Application of the PCR assay should allow more precise detection of transgenic rice varieties in imported food products which are so far not approved in the EU.

Keywords

Genetically modified organism Rice (Oryza sativa L.) Bt toxin gene Reference gene Real-time PCR Detection method 

Notes

Acknowledgements

We thank Dr. Janet Cotter (University of Exeter, Exeter, UK) for providing us with the rice sample materials. We also thank Dr. Joachim Bendiek (Bundesamt für Verbraucherschutz und Lebensmittelsicherheit, Berlin) for critical reading of the manuscript.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Dietrich Mäde
    • 1
  • Christine Degner
    • 1
  • Lutz Grohmann
    • 2
  1. 1.Landesamt für Verbraucherschutz Sachsen-AnhaltHalleGermany
  2. 2.Bundesamt für Verbraucherschutz und LebensmittelsicherheitBerlinGermany

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