European Food Research and Technology

, Volume 224, Issue 3, pp 355–364 | Cite as

Analytical characterization and the impact of ageing on anthocyanin composition and degradation in juices from five sour cherry cultivars

  • Daniel Bonerz
  • Kirsten Würth
  • Helmut Dietrich
  • Frank WillEmail author
Original Paper


Five different sour cherry cultivars were processed to sour cherry juices in order to analyze their composition and the impact of storage on anthocyanin composition. Regarding primary juice parameters all cultivars showed high dry masses (13.8–17.2° Brix) and sugar free extracts (59.4–74.3 g/L). Anthocyanins were identified as cyanidin-3-(2G-glucosylrutinoside), cyanidin-3-(2G-xylosylrutinoside), cyanidin-3-sophoroside, cyanidin-3-glucoside, cyanidin-3-rutinoside, and peonidin-3-rutinoside. Besides the original anthocyanins, 5-carboxypyrano-anthocyanins occurred as new reaction products during storage. HPLC-MSn experiments revealed, that a further ageing pigment was probably resulting from the reaction of an original anthocyanin with acetaldehyde. A significant decline of the original anthocyanin concentrations could be observed (70–75%) during 6 months of storage at 20 °C. Degradation kinetics of anthocyanins were calculated taking the reaction rate as first order. Colourless polyphenol concentrations and antioxidant capacities (TEAC) of the juices were not affected during storage.


Anthocyanins Fruit juice Polyphenols Sour cherry Storage 



This research project (AiF-Project No. 13587 N) was supported by the FEI (Forschungskreis der Ernährungsindustrie e.V., Bonn), the AiF and the Ministry of Economics and Technology.


  1. 1.
    Serafini M, Laranjinha J, Almeida L, Maiani G (2000) J Nutr Biochem 11:585–590CrossRefPubMedGoogle Scholar
  2. 2.
    Watzl B, Rechkemmer G (2001) Ernährungsumschau 48:499–503Google Scholar
  3. 3.
    Rasmussen SE, Frederiksen H, Krogholm KS, Poulsen L (2005) Mol Nutr Food Res 49:159–174CrossRefPubMedGoogle Scholar
  4. 4.
    Will F, Hilsendegen P, Bonerz D, Patz CD, Dietrich H (2005) J Appl Bot Food Qual 79:12–16Google Scholar
  5. 5.
    Will F, Dietrich H (2005) Eur Food Res Technol, DOI: 10.1007/s00217-005-0222-8Google Scholar
  6. 6.
    Zimmer E, Patz CD, Dietrich H (1992) Wein-Wissenschaft 47:121–128Google Scholar
  7. 7.
    Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans CA (1999) Free Radic Biol Med 26:1231–1237CrossRefPubMedGoogle Scholar
  8. 8.
    Singleton VL, Rossi JA (1965) Am J Enol Vitic 37:144–158Google Scholar
  9. 9.
    Will F, Banaszczyk J, Plocharski W, Dietrich H (2000) Obst-, Gemüse- Kartoffelverarbeitung 85:238–241Google Scholar
  10. 10.
    Herrmann K (1996) Ind Obst- Gemüseverw 81:121–129Google Scholar
  11. 11.
    Henning W, Herrmann K (1980) Z Lebens Unters Forsch 170:433–444CrossRefGoogle Scholar
  12. 12.
    Harborne JB, Baxter H (1999) Handbook of natural Flavonoids, vol 2. Wiley, New YorkGoogle Scholar
  13. 13.
    Hädrich J, Vogelsang J (1999) Dtsch Lebensm Rundsch 95:428–436Google Scholar
  14. 14.
    Bakker J, Bridle P, Honda T, Kuwano H, Saito N, Terahara N, Timberlake CF (1997) Phytochemistry 44:1375–1382CrossRefGoogle Scholar
  15. 15.
    Hillebrand S (2004) Analytik von Polyphenolen in Buntsäften im Hinblick auf Saftqualität, Farbe und antioxidative Aktivität. Dissertation, University of Braunschweig, Cuvillier, GöttingenGoogle Scholar
  16. 16.
    Fulcrand H, Benabdeljalil C, Rigaud J, Cheynier V, Moutounet M (1998) Phytochemistry 47:1401–1407CrossRefPubMedGoogle Scholar
  17. 17.
    Schwartz M, Quast P, von Baer D, Winterhalter P (2003) J Agric Food Chem 51:6261–6267CrossRefPubMedGoogle Scholar
  18. 18.
    Fossen T, Andersen OM (2003) Phytochemistry 62:1217–1220CrossRefPubMedGoogle Scholar
  19. 19.
    Mateus N, De Freitas VAP (2001) Food Chem 49:5217–5222CrossRefPubMedGoogle Scholar
  20. 20.
    Mateus N, Carvalho E, Carvalho ARF, Melo A, Gonzáles-Paramás AN, Santos-Buelga C, Silva AMS, De Freitas VAP (2003) J Agric Food Chem 51:277–282CrossRefPubMedGoogle Scholar
  21. 21.
    Andersen OM, Fossen T, Torskangerpoll K, Fossen A, Hauge U (2004) Phytochemistry 65:405–410CrossRefPubMedGoogle Scholar
  22. 22.
    Pesis E (2005) Postharvest Biol Technol 37(1):1–19CrossRefGoogle Scholar
  23. 23.
    Bakker J, Timberlake CF (1997) J Agric Food Chem 45:35–43CrossRefGoogle Scholar
  24. 24.
    Fossen T, Rayyan S, Andersen OM (2004) Phytochemistry 65:1421–1428CrossRefPubMedGoogle Scholar
  25. 25.
    McDougall GJ, Gordon S, Brennan RM, Stewart D (2005) J Agric Food Chem 53:7878–7885CrossRefPubMedGoogle Scholar
  26. 26.
    Boulton R (2001) Am J Enol Vitic 52:67–87Google Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Daniel Bonerz
    • 1
  • Kirsten Würth
    • 1
  • Helmut Dietrich
    • 1
  • Frank Will
    • 1
    Email author
  1. 1.Department of Wine Analysis and Beverage ResearchResearch Institute GeisenheimGeisenheimGermany

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