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European Food Research and Technology

, Volume 223, Issue 6, pp 821–827 | Cite as

Development of a rapid, simple paddle-style dipstick dye immunoassay specific for Listeria monocytogenes

  • Martina BlažkováEmail author
  • Ludmila Karamonová
  • Mária Greifová
  • Ladislav Fukal
  • Ignác Hoza
  • Pavel Rauch
  • Gary Wyatt
Original Paper

Abstract

We have developed two types of new paddle-style dipstick dye immunoassays. The first is genus Listeria specific and the second is specific to Listeria monocytogenes. They are based respectively, on antisera raised against heat-killed L. monocytogenes cells and against internalin B crude extract, a virulence protein found only in the pathogenic L. monocytogenes. The minimum detectable level for L. monocytogenes is 2×107 CFU ml−1 for strain number 88/049 in pure culture. Detection is unaffected by the presence of high numbers (approximately log 8.0 CFU/ml) of the other microorganisms tested. When the dipsticks were applied to milk samples inoculated with L. monocytogenes reference material (ALM92), there was a strong response to the enrichment cultures. The new assay may prove useful in detection of L. monocytogenes in enrichment cultures of milk and ice cream food samples.

Keywords

Dipstick immunoassay Listeria monocytogenes Milk Ice cream 

Notes

Acknowledgement

This work was supported by the Czech Grant Agency, No. 525/03/0350 and by Ministry of Education, Grant No. MSM 6046137305.

References

  1. 1.
    Farber JM, Peterkin P (2000) Listeria monocytogenes. In: Lund BM, Baird-Parker TC, Gould GW (eds) The microbiological safety and quality of food, vol II. Aspen Publishers Inc., Gaithersburg, Maryland, USA, pp 1178–1232Google Scholar
  2. 2.
    Karamonová L, Blažková M, Fukal L, Rauch P, Greifová M, Horáková K, Tomáška M, Roubal P, Brett GM, Wyatt GM (2003) Food Agric Immunol 15:167–182CrossRefGoogle Scholar
  3. 3.
    Loiseau O, Cottin J, Robert R, Tronchin G, Mahaza C, Senet JM (1995) FEMS Immunol Med Microbiol 11:219–230PubMedCrossRefGoogle Scholar
  4. 4.
    Muller S, Hain T, Pashalidis P, Lingnau A, Domann E, Chakraborty T, Wehland J (1998) Inf Immunol 66:3128–3133Google Scholar
  5. 5.
    Lingnau A, Domann E, Hudel M, Bock M, Nichterlein T, Wehland J, Chakraborty T (1995) Inf Immunol 64:3896–3903Google Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Martina Blažková
    • 1
    Email author
  • Ludmila Karamonová
    • 1
  • Mária Greifová
    • 2
  • Ladislav Fukal
    • 1
  • Ignác Hoza
    • 3
  • Pavel Rauch
    • 1
  • Gary Wyatt
    • 4
  1. 1.Department of Biochemistry and MicrobiologyInstitute of Chemical TechnologyPragueCzech Republic
  2. 2.Slovak Technical UniversityBratislavaSlovakia
  3. 3.Tomas Bata University in ZlínZlínCzech Republic
  4. 4.Institute of Food ResearchNorwich Research ParkNorwichUK

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