European Food Research and Technology

, Volume 223, Issue 1, pp 102–109 | Cite as

Phenol content related to antioxidant and antimicrobial activities of Passiflora spp. extracts

  • Alessandra Bendini
  • Lorenzo Cerretani
  • Luca Pizzolante
  • Tullia Gallina Toschi
  • Flavia Guzzo
  • Stefania Ceoldo
  • Andrea Maria Marconi
  • Filippo Andreetta
  • Marisa Levi
Original Paper

Abstract

Methanolic extracts were prepared from different organs of plants from five Passiflora species obtained by zygotic embryo culture and evaluated for their capacity to quench DPPH and ABTS•+ radicals in comparison to that of Trolox, a water soluble vitamin-E analogue. Moreover their antimicrobial activity against E. coli was tested by agar diffusion and turbidity assays. P. nitida, P. foetida, and P. palmeri showed antimicrobial activity. P. nitida and P. palmeri also showed high antioxidant activity. P. tenuifila and P. coriacea demonstrated antioxidant power but not antimicrobial activity. The phenolic content of the different extracts was studied and quantified by spectrophotometric methods, HPLC, and mass spectrometry. High antioxidant activity correlated with high amounts of o-diphenol and catechin. An unknown component, tentatively identified as structural isomer of isoschaftoside, appeared to correlate with antimicrobial activity.

Keywords

Antimicrobial activity Antioxidant activity ABTS•+ DPPH LC-MS 

Notes

Acknowledgements

M. Levi and F. Guzzo were supported by Italian MIUR (PRIN 2004) and Fondazione CARIVERONA.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Alessandra Bendini
    • 1
  • Lorenzo Cerretani
    • 1
  • Luca Pizzolante
    • 1
  • Tullia Gallina Toschi
    • 1
  • Flavia Guzzo
    • 2
  • Stefania Ceoldo
    • 2
  • Andrea Maria Marconi
    • 3
  • Filippo Andreetta
    • 2
  • Marisa Levi
    • 2
  1. 1.Dipartimento di Scienze degli AlimentiUniversità di BolognaCesenaItaly
  2. 2.Dipartimento Scientifico e TecnologicoUniversità di VeronaVeronaItaly
  3. 3.Laboratori Agroalimentari Consorzio ZAIPasqua Vigneti e CantineVeronaItaly

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