Vitamin D and provitamin D in fish

Determination by HPLC with electrochemical detection
Original Paper

Abstract

A highly-sensitive high-performance liquid chromatographic method for measuring vitamin D3 and provitamin D3 in the edible part of fish was developed. The sample preparation procedure consisted of cold saponification and liquid/liquid extraction of the unsaponifiable matter using a disposable kieselguhr cartridge. For the purification of the sample extracts a semipreparative stage using normal-phase HPLC with UV-detection was applied. A vitamin D and a provitamin D fraction was collected and subsequently quantified by analytical reversed-phase HPLC with electrochemical detection. Internal standardization was used for quantitation. The accuracy of this method was evaluated by recovery experiments. The investigated fish included most of the important fish species consumed in Germany.

Keywords

Vitamin D Provitamin D Fish Kieselguhr cartridges HPLC Electrochemical detection 

References

  1. 1.
    Combs GF (1998) In: Combs GF (ed) The vitamins, fundamental aspects in nutrition and health, 2nd edn. Academic Press, San Diego, California, pp 155–187Google Scholar
  2. 2.
    Trang HM., Cole DE, Rubin LA, Pierratos A, Siu S, Vieth R (1998) Am J Clin Nutr 68:854–858Google Scholar
  3. 3.
    Opinion of the Scientific Committee on Food on the Tolerable Upper Intake Level of Vitamin D expressed on 4.12.2002. http://europa.eu.int/comm/food/fs/sc/scf/out157_en.pdfGoogle Scholar
  4. 4.
    Barthel HR, Scharla SH (2003) Dtsch Med Wochenschr 128:440–446CrossRefGoogle Scholar
  5. 5.
    Mehta RG, Mehta RR (2002) J of Nutr Biochem 13:252–264CrossRefGoogle Scholar
  6. 6.
    Brown AJ, Dusso A, Slatopolsky E (1999) Am J Physiol 277:F157–F175Google Scholar
  7. 7.
    Holick MF (2003) J Cell Biochem 88:296–307CrossRefGoogle Scholar
  8. 8.
    D-A-CH (2000): Referenzwerte für die Nährstoffzufuhr, 1. Auflage Umschau/Braus Verlag, Frankfurt a. MainGoogle Scholar
  9. 9.
    Zittermann A (2003) Br J Nutr 89:552–572CrossRefGoogle Scholar
  10. 10.
    Rao DS, Raghuramulu N (1997) J Nutr Sci Vitaminol 43:425–433Google Scholar
  11. 11.
    Rao DS, Raghuramulu N (1999) J Nutr Sci Vitaminol 45:1–8Google Scholar
  12. 12.
    Mattila P, Piironen V, Haapala R, Hirvi T, Uusi-Rauva E (1997) J Agric Food Chem 45:3891–3896CrossRefGoogle Scholar
  13. 13.
    Eitenmiller RR, Landen WO (1998) Vitamin analysis for the health and food sciences, CRC press, pp 77–107Google Scholar
  14. 14.
    Jones G, Trafford D, Makin H, Hollis BW (1992) In: De Leenheer AP, Lambert WE, Nelis HJ (eds) Modern chromatographic analysis of vitamins, 2nd edn. Marcel Dekker, Inc., pp 73–151Google Scholar
  15. 15.
    Parrish DB (1979) CRC Critical Rev Food Sci Nutr 12:29–57CrossRefGoogle Scholar
  16. 16.
    Untersuchung von Lebensmitteln: Bestimmung von Vitamin D (Cholecalciferol (D3) und Ergocalciferol (D2)) in Lebensmitteln mittels HPLC. L 00.00-61, Juli 2001, in: Amtliche Sammlung von Untersuchungsverfahren nach §35 LMBG, Bundesgesundheitsamt (ed), Beuth, Berlin, Köln, (according to DIN EN 12821)Google Scholar
  17. 17.
    Hasegawa H (1992) J Chromatogr 605:215–220CrossRefGoogle Scholar
  18. 18.
    Masuda S, Okano T, Kamao M, Kanedai Y, Kobayashi T (1997) J Pharm Biomed Anal 15:1497–1502CrossRefGoogle Scholar
  19. 19.
    Zamarreno MMD, Pérez AS, Iglesias MCM, Méndez JH (1993) Anal Lett 26:2565–2573Google Scholar
  20. 20.
    Zamarreno MMD, Pérez AS, Pérez CG, Méndez JH (1992) J Chromatogr. 623:69–74CrossRefGoogle Scholar
  21. 21.
    ESA Application Note 70–4935, www.esainc.comGoogle Scholar
  22. 22.
    Hart JP, Norman MD, Lacey CJ (1992) Analyst 117:1441–1445CrossRefGoogle Scholar
  23. 23.
    Porteous CE, Coldwell RD, Trafford DJH, Makin HLJ (1987) J steroid Biochem 28:785-801CrossRefGoogle Scholar
  24. 24.
    Oehlenschläger J (1986) Inf Fischw 33:188–190Google Scholar
  25. 25.
    Schilling M, Armbruster FP, Schmidt-Gayk H (1987) Clin Chem 33:187Google Scholar
  26. 26.
    Scharla S, Schmidt-Gayk H, Reichel H, Mayer E (1984) Clin Chim Acta 142:323–338CrossRefGoogle Scholar
  27. 27.
    Proszynska K, Lukaszkiewicz J, Jarocewicz N, Lorenc RS (1985) Clin Chim Acta 153:85–92CrossRefGoogle Scholar
  28. 28.
    Bourgois CF, Ciba N (1988) J Assoc Off Anal Chem 71:12–15Google Scholar
  29. 29.
    Brinkmann E, Mehlitz I, Oei HB, Tiebach R, Baltes W (1994) Z Lebensm Unters Forsch 199:206–209CrossRefGoogle Scholar
  30. 30.
    Bilic N, Sieber R (1988) Fresenius Z Anal Chem 330:143–145CrossRefGoogle Scholar
  31. 31.
    Souci SW, Fachmann W, Kraut H (2000) Die Zusammensetzung der Lebensmittel, Nährwert-Tabellen, 6. Aufl., Medpharm Scientific Publishers, StuttgartGoogle Scholar
  32. 32.
    Bundeslebensmittelschlüssel, http://www.daskochrezept.de/ernaehrungGoogle Scholar
  33. 33.
    Mattila P, Piironen V, Uusi-Rauva E, Koivistoinen P (1995) J Food Comp Anal 8:232–243CrossRefGoogle Scholar
  34. 34.
    Dias MG, Sánchez MV, Bártolo H, Oliveira L (2003) Electron J Environ Agric Food Chem 2Google Scholar
  35. 35.
    Lall SP, Parazo MP (1995) In: Ruiter A (ed) Fish and fishery products. CAB International, New York, 157–186Google Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Federal Research Centre for Nutrition and FoodDepartment of Fish QualityHamburgGermany

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