Vitamin D and provitamin D in fish

Determination by HPLC with electrochemical detection
Original Paper


A highly-sensitive high-performance liquid chromatographic method for measuring vitamin D3 and provitamin D3 in the edible part of fish was developed. The sample preparation procedure consisted of cold saponification and liquid/liquid extraction of the unsaponifiable matter using a disposable kieselguhr cartridge. For the purification of the sample extracts a semipreparative stage using normal-phase HPLC with UV-detection was applied. A vitamin D and a provitamin D fraction was collected and subsequently quantified by analytical reversed-phase HPLC with electrochemical detection. Internal standardization was used for quantitation. The accuracy of this method was evaluated by recovery experiments. The investigated fish included most of the important fish species consumed in Germany.


Vitamin D Provitamin D Fish Kieselguhr cartridges HPLC Electrochemical detection 


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Federal Research Centre for Nutrition and FoodDepartment of Fish QualityHamburgGermany

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