European Food Research and Technology

, Volume 223, Issue 1, pp 22–29 | Cite as

Quantitative analysis of physical and chemical measurands in honey by mid-infrared spectrometry

  • Kaspar Ruoff
  • María Teresa Iglesias
  • Werner Luginbühl
  • Jacques-Olivier Bosset
  • Stefan Bogdanov
  • Renato Amadò
Original Paper


Fourier transform infrared spectroscopy (FT-IR) was used to determine 20 different measurands in honey. The reference values for 144 honey samples of different botanical origin were determined by classical physical and chemical methods. Partial least squares regression was used to develop the calibration models for the measurands studied. They were validated using independent samples and proved satisfying accuracies for the determination of water (R 2=0.99), glucose (0.94), fructose (0.84), sucrose (0.91), melezitose (0.98) and monosaccharide content (0.82) as well as fructose/glucose ratio (0.98), glucose/water ratio (0.94), electrical conductivity (0.98), pH-value (0.87) and free acidity (0.96). The prediction accuracy for hydroxymethylfurfural, proline and the minor sugars maltose, turanose, erlose, trehalose, isomaltose and kojibiose was rather poor. The results demonstrate that mid-infrared spectrometry is a valuable, rapid and non-destructive tool for the quantitative analysis of the most important measurands in honey.


Honey Fourier transform Infrared spectrometry FT-IR ATR Sugar 



The financial support of the Swiss Federal Office of Public Health, Narimpex AG, and the Swiss Beekeeper's Association is gratefully acknowledged. M. T. Iglesias is recipient of a doctoral grant from the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Spain).


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Kaspar Ruoff
    • 1
    • 3
  • María Teresa Iglesias
    • 2
  • Werner Luginbühl
    • 1
  • Jacques-Olivier Bosset
    • 1
  • Stefan Bogdanov
    • 1
  • Renato Amadò
    • 3
  1. 1.Swiss Bee Research Centre/Agroscope Liebefeld-PosieuxBerneSwitzerland
  2. 2.Instituto Madrileño de Investigación Agraria y Alimentaria (IMIA)Alcalá de HenaresMadridSpain
  3. 3.Swiss Federal Institute of Technology (ETH)Institute of Food Science and Nutrition, ETH-ZentrumZurichSwitzerland

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