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European Food Research and Technology

, Volume 220, Issue 2, pp 187–190 | Cite as

The effects of baobab pulp powder on the micro flora involved in tempe fermentation

  • O. R. Afolabi
  • T. O. S. Popoola
Original Paper

Abstract

Locally prepared tempe that underwent natural fermentation was characterized by the growth of Lactobacillus plantarum, Streptococcus lactis , Bacillus sp., Salmonella sp., Klebsiella sp., Lactococcus lactis , Rhizopus sp. and Staphylococcus sp., while fermentation carried out with the addition of varying levels of baobab pulp powder had mainly lactic acid bacteria (LAB)— Lactobacillus plantarum, Lactobacillus fermentum , Lactobacillus acidophilus and Rhizopus sp. dominating. Increasing concentrations of baobab pulp powder led to an increase in the population of lactic acid bacteria (LAB) from 2.3×102 to 3.3×104 while it reduced the population of inoculated Rhizopus from 102 to only six colonies on malt extract agar (MEA).

Keywords

Fermentation Tempe Baobab 

References

  1. 1.
    Egunlety M (2002) Int J Food Sci Nutr 53:15PubMedGoogle Scholar
  2. 2.
    Okon PN (1973) Characterization and biochemical studies of Adansonia digitata pulp used for ‘nono’ fermentation. MSc Thesis (Biochemistry) Ahmadu Bello University, Zaria, NigeriaGoogle Scholar
  3. 3.
    Steinkraus KH, Hand DB, Van Bureu JP, Hackler LR (1960) Pilot plant studies on tempe. In: Proceedings of Conference on Soybeans Products for Protein in Human Foods, USDA, pp 75–84Google Scholar
  4. 4.
    Liem ITH, Steinkraus KH, Crouk TC (1977) Production of vitamin B-12 in tempe, a fermented soybean food. App 1. Environ Microbiol 34:773–776Google Scholar
  5. 5.
    Wang HL, Hesseltine CW (1981) Use of microbial cultures: legume and cereal products. Food Technol 35:79–83Google Scholar
  6. 6.
    Beuchat LR (1983) Indigenous fermented foods In: Reed G (ed) Biotechnology, vol.5: food and feed production with microorganisms. Verlag Chemie, Weinheim, pp 477–528Google Scholar
  7. 7.
    Beuchat LR (1984) Fermented soybean foods. Food Technol 38:64–70Google Scholar
  8. 8.
    Steinkraus KH (1983) Trend and current knowledge in tempe research. Proceedings from the symposium on the utilization of tempe for the improvement of health and nutrition, Jakarta, Indonesia. pp 138–148Google Scholar
  9. 9.
    Hachmeister KA, Fung DYC (1993) Tempe: a mold modified indigenous fermented food made from soybean and/or cereal grain. Crit Rev Microbiol 19(3):137–188PubMedGoogle Scholar
  10. 10.
    Sudarmadji S, Markakis P (1978) Lipid and other changes occurring during the fermentation and frying of tempe. Food Chem 3:165–170CrossRefGoogle Scholar
  11. 11.
    Nout MJR, Bonants-Van Laarhoven TMG, de Draux R, Gerats IAGM (1985) The influence of source process variables and storage conditions on the quality and shelf life of soybean tempe. Antonie van Leeuwenhoek 51:532–534Google Scholar
  12. 12.
    Samson RA, Van Kooji JA, Beboer E (1987) Microbiological quality of commercial tempe in the Netherlands. J Food Protect 50:92–94Google Scholar
  13. 13.
    Nout MJR, De Dren MA, Zuurbier AM, Bonants van Laarhoven TMG (1987) Ecology of controlled soybean acidification for tempe manufacture. Food Microbiol 4:165–172Google Scholar
  14. 14.
    Tanaka N, Kovats SK, Guggisberg JA, Meske LM, Doyle MP (1985) Evaluation of the microbiological safety of tempe made from unacidified soybeans. J Food Protect 48:438–441Google Scholar
  15. 15.
    Nout MJR, Beernink G, Bonants-van Laarhoven TMG (1987a) Growth of Bacillus cereus in soybean tempe. Int J Food Microbiol 4:293–301CrossRefGoogle Scholar
  16. 16.
    Robert KM, Graham HF, Ken AB (1989) The microbial ecology of soybean soaking for tempe production. Int J Food Microbiol 8:35–46CrossRefPubMedGoogle Scholar
  17. 17.
    Kandler O, Weiss N (1986) Regular, non-sporing gram-positive rods. In: Holt J (ed) Bergey’s manual of systematic bacteriology, vol. 2. William and Wilkins, BaltimoreGoogle Scholar
  18. 18.
    Thomas JM, Ciurana B, Jofre JT (1986) New, simple medium for selective, differential recovery of Klebsiella sp. Appl Environ Microbiol 51:1361–1363PubMedGoogle Scholar
  19. 19.
    Cowan ST, Steel JK (1970) The identification of medical bacteria. Cambridge University Press, LondonGoogle Scholar
  20. 20.
    Krieg NR, Holt JG (1984) Bergey’s manual of systematic bacteriology, vol. 1. Williams and Wilkins, BaltimoreGoogle Scholar
  21. 21.
    Sneath PHA, Mair NS, Sharpe ME, Holt JG (1986) Bergey’s manual of systematic bacteriology, vol 2. Williams and Wilkins, BaltimoreGoogle Scholar
  22. 22.
    Sanni AI, Ahrne S, Onilude AA (1995) Production of (- galactosidase by Lactobacillus plantarum isolated from diverse sources. J Basic Microbiol 35:427–432Google Scholar
  23. 23.
    Sanni AI, Onilude AA, Ogundoye OR (1997) Effect of bacteria galactosidases treatment on the nutritional status of soybean seeds and its milk derivative. Nahrung Foods 41:18–21Google Scholar
  24. 24.
    Sanni AI, Onilude AA, Ogunbanwo ST, Fadahunsi IF, Afolabi RO (2002) Eur Food Res Technol 215:176–180CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Department of Microbiology, College of Natural SciencesUniversity of AgricultureAbeokutaNigeria

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