European Food Research and Technology

, Volume 217, Issue 2, pp 180–184 | Cite as

Comparison of methods for determining malondialdehyde in dry sausage by HPLC and the classic TBA test

  • Anna de las Heras
  • Alexander Schoch
  • Monika Gibis
  • Albert Fischer
Original Paper


A reproducible and sensitive HPLC assay with fluorometric detection for measuring malondialdehyde as an index of lipid peroxidation in dry sausage (salami) has been developed. Following the homogenisation of dry sausage in water, a solution of 5% ice-cooled trichloroacetic acid led to the precipitation of the proteins. The addition of thiobarbituric acid (TBA) (0.02 M) to the filtered mixture and its corresponding incubation (35 min, 100 °C) completed the method. High correlation coefficients (r>0.977), with regard to the distillative TBA test, were obtained. Ratios between the HPLC and the distillation method of 2.23 were found. Also, the effect of the antioxidant BHT and an examination of the distillative classic TBA test have been done. Results indicate that this HPLC method, on account of its simplicity, sensitivity and specificity, may be preferred over the classic TBA test for estimating the rancidity of dry sausage.


Malondialdehyde Lipid peroxidation High performance liquid chromatography Thiobarbituric acid test 


  1. 1.
    Sinnhuber R, Yu TC (1958) Food Technol 12:9–12Google Scholar
  2. 2.
    Tarladgis BG, Watts BM, Younathan MT, Dugan L (1960) J Am Oil Chem Soc 37:44–48Google Scholar
  3. 3.
    Kosugi H, Kato T, Kikugawa K (1987) Anal Biochem 165:456–464PubMedGoogle Scholar
  4. 4.
    Kosugi H, Kato T, Kikugawa K (1988) Lipids 23:1024–1031Google Scholar
  5. 5.
    Kosugi H, Kojima T, Kikugawa K (1989) Lipids 24:873–881Google Scholar
  6. 6.
    Janero DR (1990) Free Radical Biol Med 9:515–540CrossRefGoogle Scholar
  7. 7.
    Shamberger RJ, Shamberger BA, Willis CE (1977) J Nutr 107:1404–1409PubMedGoogle Scholar
  8. 8.
    Squires EJ (1990) Poult Sci 69:1371–1376PubMedGoogle Scholar
  9. 9.
    Kakuda Y, Stanley DW, Voort FR (1981) J Am Oil Chem Soc 58:773–775Google Scholar
  10. 10.
    Tsaknis J, Lalas S, Evmorfopoulos E (1999) Analyst 124:843–845CrossRefPubMedGoogle Scholar
  11. 11.
    Bergamo P, Fedele E, Balestrieri M, Abrescia P, Ferrara L (1998) J Agric Food Chem 46:2171–2176CrossRefGoogle Scholar
  12. 12.
    Jo C, Ahn DU (1998) Poult Sci 77:475–480PubMedGoogle Scholar
  13. 13.
    Zipser MW, Watts B W (1962) Food Technol 16:102–104Google Scholar
  14. 14.
    Kwon T, Menzel DB, Olcott HS (1965) J Food Sci 30:808–813Google Scholar
  15. 15.
    Draper HH, McGirr LG, Hadley M (1986) Lipids 21:305–307PubMedGoogle Scholar
  16. 16.
    Botsoglou NA, Fletouris DJ, Papageorgiou GE, Vassilopoulos VN, Mantis AJ, Trakatellis AG (1994) J Agric Food Chem 42:1931–1937Google Scholar
  17. 17.
    Wang C, Zhu L, Brewer MS (1997) J Food Lipids 4:87–96Google Scholar
  18. 18.
    Pikul J, Leszcezynski DE, Kummerow FA (1989) J Agric Food Chem 37:1309–1313Google Scholar
  19. 19.
    Raharjo S, Sofos JN, Schmidt GR (1993) J Food Sci 58:921–924Google Scholar
  20. 20.
    Siu GM, Draper HH (1978) J Food Sci 43:1147–1149Google Scholar
  21. 21.
    Rhee K (1978) J Food Sci 43:1776–1779Google Scholar
  22. 22.
    Pikul J, Leszczynski DE, Kummerow FA (1989) J Agric Food Chem 37:1309–1313Google Scholar
  23. 23.
    Ke PJ, Cervantes E, Robles-Martinez C (1984) J Sci Food Agric 35:1248–1254Google Scholar
  24. 24.
    Salih AM, Smith DM, Dawson LE (1987) Poult Sci 66:1483–1488PubMedGoogle Scholar
  25. 25.
    Salih AM, Price JF, Smith DM, Dawson LE (1989) Poult Sci 68:754–761Google Scholar
  26. 26.
    Caldironi HA, Bazan NG (1982) J Food Sci 47:1329–1332, 1337Google Scholar
  27. 27.
    Spanier AM, Traylor RD (1991) J Muscle Foods 2:165–175Google Scholar
  28. 28.
    Moerck KE, Ball HR (1974) J Food Sci 39:876–879Google Scholar
  29. 29.
    Shahidi F, Hong C (1991) J Food Biochem 15:97–105Google Scholar
  30. 30.
    Hoyland DV, Taylor AJ (1991) Food Chem 40:271–291Google Scholar
  31. 31.
    Karastogiannidou C, Ryley J (1994) Int J Food Sci Technol 29:19–22Google Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Anna de las Heras
    • 1
  • Alexander Schoch
    • 1
  • Monika Gibis
    • 1
  • Albert Fischer
    • 1
  1. 1.Institute of Food Technology, Department of Meat TechnologyUniversity of HohenheimStuttgartGermany

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