Effect of gamma irradiation on flavonoids in strawberries
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In unirradiated strawberries, in addition to ellagic acid, five flavonoids were identified by comparison to standards by reversed-phase chromatography with diode-array detection: (+)-catechin, (–)-epicatechin, kaempferol-3-glucoside, quercetin-3-glucoside and quercetin-3-galactoside. Four others were classified on the basis of UV spectra: two as kaempferol-, one as quercetin- and one as ellagic acid derivatives. Whereas the concentrations of the quercetin- and ellagic acid derivatives were not affected by irradiation, those of the catechin and kaempferol components diminished noticeable. The most significant reduction as a function of dose was found for (+)-catechin, followed by kaempferol-3-glucoside and (–)-epicatechin. Their decreases are, however, in the same order of magnitude as the variation of their concentrations in different harvests and varieties. For two as yet unclassified compounds, characteristic changes upon radiation were observed. The level of one of them is almost quintupled at a dose of 6 kGy.
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