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Fresenius’ Journal of Analytical Chemistry

, Volume 371, Issue 6, pp 775–781 | Cite as

Two-dimensional gel electrophoresis of selenized yeast and autoradiography of 75Se-containing proteins

  • C. Chéry
  • E. Dumont
  • R. Cornelis
  • L. Moens
Special Issue Paper

Abstract

Two-dimensional high-resolution gel electrophoresis (2DE) has been applied to the fractionation of 75Se-containing proteins in yeast, grown in 75Se-containing medium, and autoradiography was used for detection of the 75Se-containing proteins. Gel filtration and ultrafiltration were used to check whether the selenium side-chains were stable in the presence of the chemicals used for lysis and 2DE. The mass distribution of the selenium-containing proteins was estimated by use of gel filtration and the results were compared with the distribution obtained by 2DE. A 2DE map of selenium-containing proteins in yeast is presented, and compared with a total protein map of yeast.

Keywords

Selenium Selenite Selenocysteine Selenium Compound Iodoacetic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • C. Chéry
    • 1
  • E. Dumont
    • 1
  • R. Cornelis
    • 1
  • L. Moens
    • 1
  1. 1.Laboratory of Analytical Chemistry, Ghent University, Proeftuinstraat 86, 9000 GhentBelgium

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