Fresenius' Journal of Analytical Chemistry

, Volume 371, Issue 2, pp 238–245

Slide-format proteomic biochips based on surface-enhanced nanocluster-resonance

  • C. Mayer
  • N. Stich
  • T. Schalkhammer
  • G. Bauer
Special Issue Paper

Abstract.

The most fundamental properties of metal nanoclusters, namely the high local-field enhancement and nanoscale resonance behavior of the cluster electron plasma when exited by electromagnetic radiation, have been used to set up a variety of sensors transducing biorecognitive interactions into optical signals. This paper focuses on applications of resonant-cluster technology, which enabled us to monitor biorecognitive binding of a variety of proteins on a chip, thus constructing high-throughput interaction-screening devices.

Decisive for this type of sensor is the nanometric distance from the local field surrounding a cluster to other parts of the sensor interacting with this field. In particular, the cluster–mirror or cluster–fluorophore distance gives rise to a variety of enhancement phenomena. Depending on the desired application this "resonance" – distance is approximately 5–400 nm.

All types of sensor can be set up on photolithographically constructed microchips, but microscopic glass slides can also be employed; this also enables the use of standard devices for dotting and read out.

Using slide based chips a standard format of 3200 microdots (125 µm in diameter) was the basis of either microassays applying direct optical transduction via surface-enhanced absorption or striking for more sensitivity via surface-enhanced fluorescence.

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • C. Mayer
    • 1
  • N. Stich
    • 1
  • T. Schalkhammer
    • 1
  • G. Bauer
    • 2
  1. 1.Institute of Analytical Biotechnology, Technical University Delft, Julianalaan 67, 2628 BC Delft, The NetherlandsThe Netherlands
  2. 2.Institute of Biochemistry, University of Vienna, Vienna, AustriaAustria
  3. 3.November AG, Erlangen, GermanyGermany

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