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Fresenius' Journal of Analytical Chemistry

, Volume 370, Issue 7, pp 930–934 | Cite as

Application of a novel micro-injector in the determination of indole derivatives in the rat pineal gland by capillary electrophoresis with electrochemical detection

  • Gang Chen
  • Jieshi Cheng
  • Jiannong Ye
Original paper

Abstract

A novel micro-injector has been fabricated for capillary electrophoresis (CE). It was successfully used for the determination of some indole derivatives for example melatonin (MT), serotonin (5-HT), tryptophan (Trp), and 5-hydroxy-tryptophane (5-HTrp) in the rat pineal gland by capillary electrophoresis with electrochemical detection (CE–EC). CE was performed in 0.20 mol L–1 phosphate buffer (pH 8.0). The compounds investigated can be well separated and detected within 15 min. The working electrode used was a 300-μm diameter carbon electrode positioned opposite the outlet of the capillary. The relationship between peak current and analyte concentration was highly linear in the range from 0.10 to 500 μmol L–1; detection limits (S/N = 3) were 0.03–0.13 μmol L–1. The proposed method has been successfully used to analyze real biological samples.

Keywords

Phosphate Buffer Detection Limit Electrophoresis Serotonin Tryptophan 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • Gang Chen
    • 1
  • Jieshi Cheng
    • 2
  • Jiannong Ye
    • 3
  1. 1.Department of Chemistry, National Key Laboratory of Medical Neurobiology, Medical Center of Fudan University, Shanghai 200032, P. R. China e-mail: g-chen@eastday.comCN
  2. 2.Department of Medical Neurobiology, National Key Laboratory of Medical Neurobiology, Medical Center of Fudan University, Shanghai 200032, P. R. China e-mail: jscheng@shmu.edu.cnCN
  3. 3.Department of Chemistry, East China Normal University, Shanghai 200062, P. R. China e-mail: jnye@ecnu.edu.cn, Tel.: +86-021-62232254, Fax: +86-021-62576217CN

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