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Fresenius' Journal of Analytical Chemistry

, Volume 366, Issue 6–7, pp 622–634 | Cite as

Electrochemical immunoassays

  • A. Warsinke
  • A. Benkert
  • F. W. Scheller
Review

Abstract

Immunoassays (IA) use the specific antigen antibody complexation for analytical purposes. Radioimmunoassays (RIA), fluorescence immunoassays (FIA) and enzyme immunoassays (EIA) are well established in clinical diagnostics. For the development of hand-held devices which can be used for point of care measurements, electrochemical immunoassays are promising alternatives to existing immunochemical tests. Moreover, for opaque or optically dense matrices electrochemical methods are superior. Potentiometric, capacitive and amperometric transducers have been applied for direct and indirect electrochemical immunoassays. However, due to their fast detection, broad linear range and low detection limit, amperometric transducers are preferred. Competitive and non-competitive amperometric immunoassays have been developed with redox compounds or enzymes as labels.

This review will give an overview of the most frequently applied principles in electrochemical immunoassays. The potential of an indirect competitive amperometric immunoassay for the determination of creatinine within nanomolar range and the circumvention of the most serious problem in electrochemical immunoassays, namely regeneration, will be discussed.

Keywords

Dense Matrice Enzyme Immunoassay Specific Antigen Electrochemical Method Care Measurement 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • A. Warsinke
    • 1
  • A. Benkert
    • 1
  • F. W. Scheller
    • 1
  1. 1.University of Potsdam, Institute of Biochemistry and Molecular Physiology, Im Biotechnologiepark, 14943 Luckenwalde, Germany e-mail: warsinke@rz.uni-potsdam.deDE

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