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Fresenius' Journal of Analytical Chemistry

, Volume 364, Issue 4, pp 338–346 | Cite as

Multielement analysis of whole blood by high resolution inductively coupled plasma mass spectrometry

  • I. Rodushkin
  • Fredrik Ödman
  • Stefan Branth
Original paper

Abstract

An analytical method using double focusing sector field inductively coupled plasma mass spectrometry (ICP-SMS) for rapid simultaneous determination of 50 elements in digested human blood is described. Sample preparation consisted of microwave digestion with nitric acid followed by dilution with ultrapure water. The importance of controlling possible contamination sources at different sample preparation and analysis stages in order to achieve adequate method detection limits (MDL) is emphasised. Correction for matrix effects was made using scandium, indium and lutecium as internal standards. Accuracy of the data for elements suffering from spectral interferences was improved by applying either a high resolution capability of the ICP-SMS or mathematical corrections. Different approaches for accuracy assessment in blood analysis are evaluated. Additional information on trace elements concentration in selected blood reference materials is given. The between-batch precision was assessed from replicate analysis (including sample preparation) of reference materials and was better than 10% RSD for 21 elements and better than 30% RSD for 36 elements under consideration. A statistical summary for results obtained for 31 blood samples from non-exposed subjects is presented. The majority of elements tested was found in the samples at concentrations higher than MDL.

Keywords

Inductively Couple Plasma Mass Spectrometry Scandium Trace Element Concentration Accuracy Assessment Microwave Digestion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • I. Rodushkin
    • 1
  • Fredrik Ödman
    • 1
  • Stefan Branth
    • 2
  1. 1.Svensk Grundämnesanalys AB, Luleå University of Technology, S-971 87 Luleå, SwedenSE
  2. 2.Department of Medical Sciences, Nutrition, Uppsala University, S-752 37 Uppsala, SwedenSE

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