Fresenius' Journal of Analytical Chemistry

, Volume 359, Issue 2, pp 150–154

Novel optical pH-sensor based on a boradiaza-indacene derivative

  • T. Werner
  • C. Huber
  • S. Heinl
  • M. Kollmannsberger
  • J. Daub
  • O. S. Wolfbeis
Original paper

Abstract

A pH-optode is described based on 1, 3, 5, 7-tetramethyl-8-(4-dimethylamino)-4-difluorobora-3a, 4a-diaza-(s)-indacene (1) immobilized in a hydrogel matrix. Its uncharged form is non-fluorescent, whereas the protonated form displays strong emission. The quenching of the emission can be mechanistically explained by a PET (photoinduced electron transfer) from the dimethylamino group to the fluorophore. The perpendicular configuration at the C(8)-aryl bond contributes to the efficiency of the on/off-switching behavior. Incorporation of the dye into a hydrogel matrix results in a transparent sensor membrane which can be used to measure the pH in the range of 1-3. The sensor shows high signal intensity, good reproducibility, and the response characteristic is stable over hours of operation even at low pH-values.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • T. Werner
    • 1
  • C. Huber
    • 1
  • S. Heinl
    • 2
  • M. Kollmannsberger
    • 2
  • J. Daub
    • 2
  • O. S. Wolfbeis
    • 1
  1. 1.Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, D-93040 Regensburg, GermanyDE
  2. 2.Institute of Organic Chemistry, University of Regensburg, D-93040 Regensburg, GermanyDE

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