Fresenius' Journal of Analytical Chemistry

, Volume 359, Issue 1, pp 83–87

Ex-vivo determination of blood glucose by microdialysis in combination with infrared attenuated total reflection spectroscopy

  • H. M. Heise
  • A. Bittner
  • T. Koschinsky
  • F. A. Gries
Lecture

Abstract

Several biosensors have been developed for continuous monitoring of human blood glucose, which is desirable for insulin-dependent diabetic patients. Developments in the field of quantitative assays using infrared attenuated total reflection spectroscopy allow the determination of metabolites at low concentrations. The microdialysis technique can provide a continuous sampling of extracellular body fluids. As only compounds of low molecular weight are passed on, infrared spectrometric quantitation is eased considerably. Samples were obtained by microdialysis of human blood plasma and aqueous glucose solutions. Multivariate calibration by partial least-squares was evaluated for its analytical performance in ex-vivo blood glucose monitoring. Mean squared prediction errors obtained by cross validation were 5.4 mg/dL for dialysate samples from different patients and 1.3 mg/ dL for dialysates from glucose solutions. Further investigations were carried out to achieve miniaturization of the measuring and detection device.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • H. M. Heise
    • 1
  • A. Bittner
    • 1
  • T. Koschinsky
    • 2
  • F. A. Gries
    • 2
  1. 1.Institut für Spektrochemie und Angewandte Spektroskopie, Bunsen-Kirchhoff-Strasse 11, D-44139 Dortmund, GermanyDE
  2. 2.Diabetes-Forschungsinstitut an der Universität Düsseldorf, Auf’m Hennekamp 65, D-40225 Düsseldorf (Germany)DE

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