Fresenius' Journal of Analytical Chemistry

, Volume 367, Issue 4, pp 352–355 | Cite as

Imaging the activity of nitrate reductase by means of a scanning electrochemical microscope

  • J. Zaumseil
  • G. Wittstock
  • S. Bahrs
  • P. Steinrücke
Special Issue Paper

Abstract

Scanning electrochemical microscopy (SECM) was used to characterize immobilized nitrate reductase (NaR) from Pseudomonas stutzeri (E.C. 1.7.99.4). Nitrate reductase with membrane fragment was embedded in a polyurethane hydrogel in a capillary and solubilized NaR without membrane fragment was covalently coupled to a diaminoethyl-cellulose-carbamitate film on glass. After systematic studies of possible mediators, SECM feedback imaging of both forms of immobilized NaR was accomplished with methylviologen as redox mediator.

Keywords

Nitrate Microscopy Pseudomonas Polyurethane Systematic Study 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • J. Zaumseil
    • 1
  • G. Wittstock
    • 1
  • S. Bahrs
    • 2
  • P. Steinrücke
    • 2
  1. 1.Wilhelm-Ostwald-Institute of Physical and Theoretical Chemistry, University of Leipzig, Linnéstrasse 2, D-04103 Leipzig, GermanyDE
  2. 2.Institute of Molecular Biotechnology, Beutenbergstrasse 11, D-07745 Jena, GermanyDE

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