Metabolomic profile perturbations of serum, lung, bronchoalveolar lavage fluid, spleen and feces in LPS-induced acute lung injury rats based on HPLC-ESI-QTOF-MS

  • Tianyang Wang
  • Song Lin
  • Ran Liu
  • Hua Li
  • Zihan Liu
  • Xinnong Zhang
  • Huarong Xu
  • Qing Li
  • Kaishun BiEmail author
Research Paper


Acute lung injury (ALI) is a clinically common and serious disease, underscoring the urgent need for clarification of its pathogenesis. According to traditional Chinese medicine (TCM) theories on the “lung–spleen–intestine axis” and its correlation with ALI, a high-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (HPLC–QTOF-MS) metabolomic platform was applied to identify biomarkers from five bio-samples of control and model rats challenged with intratracheally administered lipopolysaccharide (LPS) based on multivariate mathematical statistical analysis. As a result, 19, 24, 24, 15 and 29 altered metabolites were identified in serum, lung, bronchoalveolar lavage fluid (BALF), spleen and feces samples, respectively. Metabolic pathway analysis showed that linoleic acid, sphingolipid, glycerophospholipid and bile acid metabolism pathways were mainly altered by ALI. Additionally, ROC curves were applied to assess the specificity and sensitivity of the biomarkers. ALI characteristic metabolomic spectra were then established to differentiate the control from the model group with a similarity discriminative threshold of 0.7. Additionally, to compare the metabolomic profiles of the five bio-samples and establish metabolic similarities and differences among them, correlation analysis was conducted in order to delineate an objective law of endogenous linkage along the lung–spleen–intestine axis. Therefore, this study provides insights into the mechanisms involved in ALI from a metabolomics perspective, which can be applied in characterization of the mechanism and early disease detection.

Graphical abstract


Acute lung injury HPLC-QTOF-MS Metabolomics Biomarker 


Funding information

This study was supported by the National Natural Science Foundation of China (U1508220); Liaoning Distinguished Professor Project for Qing Li (2017), National Natural Science Funds of China (Grant No. 81973464), Natural Science Foundation of Liaoning Province of China (Grant No.2018010961-301), and Key R&D Program of Liaoning Province (2018226003).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2019_2357_MOESM1_ESM.pdf (2.5 mb)
ESM 1 (PDF 2.46 MB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2020

Authors and Affiliations

  • Tianyang Wang
    • 1
  • Song Lin
    • 2
  • Ran Liu
    • 1
  • Hua Li
    • 1
  • Zihan Liu
    • 1
  • Xinnong Zhang
    • 3
  • Huarong Xu
    • 1
  • Qing Li
    • 1
  • Kaishun Bi
    • 1
    Email author
  1. 1.School of PharmacyShenyang Pharmaceutical UniversityShenyangChina
  2. 2.Basic Medical Science CollegeQiqihar Medical UniversityQiqiharChina
  3. 3.School of Traditional Chinese Materia MedicaShenyang Pharmaceutical UniversityShenyangChina

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