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Evaluation of the bioaccumulation potential of alizarin red S in fish muscle tissue using the European eel as a model

Abstract

For fish stock management and large-scale stocking programs, the chemical substance alizarin red S (ARS) is an important tool to mark fish permanently. Equally, for the IUCN red list species European eel (Anguilla anguilla), ARS is proven to be the most promising option for mass marking. ARS binds to calcified structures (i.e., bones and otoliths) and can be detected using a fluorescence microscope. Despite the frequent application of ARS, not only for eels but also for fish in general, until today, no study has evaluated its bioaccumulation potential. Therefore, the German Federal Risk Assessment Authority was unable to classify ARS as harmless because of a potential risk to consumers’ health. Using the technique of liquid chromatography mass spectrometry, an ARS detection protocol was developed and the bioaccumulation potential of ARS in European eel muscle tissue was estimated. A detection limit of 8.9 μg kg−1 could be reached by optimizing the detection method in fish muscle tissue. In the current study, 250 eels between 6 and 57 cm of total length have been analyzed for ARS between 0 day and 3 years after the marking process. The highest concentration of ARS (6056 μg kg−1) was observed immediately after marking in the smallest length class. Only 1 year after the marking procedure, the ARS concentration was below detection limit. A new method for ARS detection in fish muscle tissue, followed by utilization on marked eels, was able to show that the bioaccumulation of ARS in edible fish muscle was highly unlikely.

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Acknowledgments

The authors wish to thank Dr. T. Schaarschmidt for establishing contact with the Department of Residue Analysis of the State Office for Agriculture, Food Safety and Fisheries.

Funding

This study was partly funded by the European Maritime Fisheries Fund (EMFF) and the Federal State Mecklenburg-Western Pomerania.

Author information

Correspondence to Laura Kullmann.

Ethics declarations

Prior to the study, the responsible Animal Protection Authority (State Office for Agriculture, Food Safety and Fisheries, Thierfelderstraße 18, 18059 Rostock, Germany) of the state Mecklenburg-Western Pomerania was contacted. Based on the consultation procedure, it was established that the present study complies with the Animal Welfare Regulation according to the national guidelines (Law on Animal Welfare §7a paragraph (2)3).

Conflict of interest

The authors declare that they have no conflict of interest.

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Kullmann, L., Habedank, F., Kullmann, B. et al. Evaluation of the bioaccumulation potential of alizarin red S in fish muscle tissue using the European eel as a model. Anal Bioanal Chem 412, 1181–1192 (2020). https://doi.org/10.1007/s00216-019-02346-4

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Keywords

  • Anguilla anguilla
  • Alizarin red S
  • LC-MS/MS
  • ARS concentration
  • Bioaccumulation potential
  • Fish marking