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Analytical and Bioanalytical Chemistry

, Volume 411, Issue 29, pp 7907–7913 | Cite as

SERS assessment of the cancer-specific methylation pattern of genomic DNA: towards the detection of acute myeloid leukemia in patients undergoing hematopoietic stem cell transplantation

  • Vlad Moisoiu
  • Andrei Stefancu
  • Stefania D. Iancu
  • Tudor Moisoiu
  • Luminita Loga
  • Lucia Dican
  • Cristian D. Alecsa
  • Imre Boros
  • Anca Jurj
  • Delia Dima
  • Cristina Bagacean
  • Romulus Tetean
  • Emil Burzo
  • Ciprian Tomuleasa
  • Florin Elec
  • Nicolae LeopoldEmail author
Research Paper

Abstract

In this label-free surface-enhanced Raman scattering (SERS) study of genomic DNA, we demonstrate that the cancer-specific DNA methylation pattern translates into specific spectral differences. Thus, DNA extracted from an acute myeloid leukemia (AML) cell line presented a decreased intensity of the 1005 cm−1 band of 5-methylcytosine compared to normal DNA, in line with the well-described hypomethylation of cancer DNA. The unique methylation pattern of cancer DNA also influences the DNA adsorption geometry, resulting in higher adenine SERS intensities for cancer DNA. The possibility of detecting cancer DNA based on its SERS spectrum was validated on peripheral blood genomic DNA samples from n = 17 AML patients and n = 17 control samples, yielding an overall classification of 82% based on the 1005 cm−1 band of 5-methylcytosine. By demonstrating the potential of SERS in assessing the methylation status in the case of real-life DNA samples, the study paves the way for novel methods of diagnosing cancer.

Graphical abstract

Keywords

Acute myeloid leukemia 5-Methylcytosine SERS DNA methylation Epigenetics 

Notes

Acknowledgments

A.S. and S.I. acknowledge support from the College for Advanced Performance Studies, Babeș-Bolyai University, Cluj-Napoca, Romania.

Funding information

This work was supported by a grant from the Romanian Ministry of Research and Innovation, CCCDI-UEFISCDI, project number PN-III-P4-ID-PCCF-2016-0112 within PNCDI III.

Compliance with ethical standards

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Conflict of interest

The authors declare that they have no conflict of interest.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

216_2019_2213_MOESM1_ESM.pdf (438 kb)
ESM 1 (PDF 691 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Vlad Moisoiu
    • 1
    • 2
  • Andrei Stefancu
    • 1
  • Stefania D. Iancu
    • 1
  • Tudor Moisoiu
    • 3
  • Luminita Loga
    • 3
  • Lucia Dican
    • 4
  • Cristian D. Alecsa
    • 5
    • 6
  • Imre Boros
    • 5
    • 6
  • Anca Jurj
    • 7
  • Delia Dima
    • 8
  • Cristina Bagacean
    • 9
  • Romulus Tetean
    • 1
  • Emil Burzo
    • 1
  • Ciprian Tomuleasa
    • 7
    • 8
    • 10
  • Florin Elec
    • 3
    • 11
  • Nicolae Leopold
    • 1
    Email author
  1. 1.Faculty of PhysicsBabeș-Bolyai UniversityCluj-NapocaRomania
  2. 2.Faculty of MedicineIuliu Hatieganu University of Medicine and PharmacyCluj-NapocaRomania
  3. 3.Clinical Institute of Urology and Renal TransplantCluj-NapocaRomania
  4. 4.Department of BiochemistryIuliu Hatieganu University of Medicine and PharmacyCluj-NapocaRomania
  5. 5.Faculty of Mathematics and Computer ScienceBabeș-Bolyai UniversityCluj-NapocaRomania
  6. 6.Tiberiu Popoviciu Institute of Numerical AnalysisCluj-NapocaRomania
  7. 7.Research Center for Functional Genomics and Translational Medicine/HematologyIuliu Hatieganu University of Medicine and PharmacyCluj NapocaRomania
  8. 8.Department of HematologyIon Chiricuta Clinical Cancer CenterCluj-NapocaRomania
  9. 9.INSERM UMR1227, B Lymphocytes and AutoimmunityUniversity Bretagne OccidentaleBrestFrance
  10. 10.Department of HematologyIuliu Hatieganu University of Medicine and PharmacyCluj-NapocaRomania
  11. 11.Department of UrologyIuliu Hatieganu University of Medicine and PharmacyCluj-NapocaRomania

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