Leek-derived codoped carbon dots as efficient fluorescent probes for dichlorvos sensitive detection and cell multicolor imaging

  • Yuefang HuEmail author
  • Jinfang LiEmail author
  • Xuefeng Li
Research Paper


A biomass nitrogen and sulfur codoped carbon dots (NS-Cdots) was prepared by a simple and clean hydrothermal method using leek, and was employed as efficient fluorescent probes for sensitive detection of organophosphorus pesticides (OPs). The leek-derived NS-Cdots emitted blue fluorescence, but was quenched by H2O2. Due to acetylcholinesterase/choline oxidase–based cascade enzymatic reaction that produces H2O2 and the inhibition effect of OPs on acetylcholinesterase activity, a NS-Cdots-based fluorescence “off-on” method to detect OPs-dichlorvos (DDVP) was developed. More sensitivity and wider linear detection range were achieved from 1.0 × 10−9 to 1.0 × 10−3 M (limit of detection = 5.0 × 10−10 M). This developed method was applied to the detection of DDVP in Chinese cabbage successfully. The average recoveries were in the range of 96.0~104.0% with a relative standard deviation of less than 3.3%. In addition, the NS-Cdots fluorescent probes were also employed successfully in multicolor imaging of living cells, manifesting that the NS-Cdots fluorescent probes have great application potential in agricultural and biomedical fields.

Graphical Abstract


Codoped carbon dots Organophosphorus pesticides Fluorescence detection Cells multicolor imaging 


Funding information

This study was funded by the Natural Science Foundation of Guangxi Province (No. 2017GXNSFAA198274)

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards and informed consent

This research was approved by the Hezhou University Ethic Committee and all experiments were performed in accordance with the Guideline for Experimentation of Hezhou University.

Supplementary material

216_2019_2192_MOESM1_ESM.pdf (702 kb)
ESM 1 (PDF 702 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Materials and Chemical EngineeringHezhou UniversityHezhouChina
  2. 2.College of Chemistry and Pharmaceutical SciencesGuangxi Normal UniversityGuilinChina

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