Defining a system suitability limit to decide on column deterioration and to facilitate column transfers in chiral supercritical fluid chromatography

  • Sven Declerck
  • Yvan Vander Heyden
  • Debby MangelingsEmail author
Research Paper
Part of the following topical collections:
  1. Female Role Models in Analytical Chemistry


The separation of enantiomers is an important requirement during the entire drug life cycle in the pharmaceutical industry. High-performance liquid chromatography and supercritical fluid chromatography (SFC) are the main chromatographic techniques used to separate enantiomers. Since chiral stationary phases are often extensively used once a method has been developed, columns will age and must be replaced after a certain period. However, no practical guidelines exist to determine when a column is deteriorated or to decide whether a transfer to another column (with the same chiral selector) is successful. In this study, a system suitability limit for resolution was defined, based on an intermediate (time-different) precision study in SFC on four immobilized polysaccharide-based columns that only differed in manufacturer or particle size. This system suitability limit could be used to decide on column deterioration or as a requirement to evaluate whether a separation transfer was successful. Some method adaptations may be necessary to obtain successful transfers. An approach was proposed, which helped the analyst to make successful transfers.

Graphical abstract


Supercritical fluid chromatography Enantioseparations Intermediate-precision study System suitability Transfer 



The authors would like to thank Ana Ocaña for performing a part of the experiments during her Erasmus stay. The authors are grateful to Phenomenex and YMC for providing columns.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2019_2173_MOESM1_ESM.pdf (118 kb)
ESM 1 (PDF 118 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Sven Declerck
    • 1
  • Yvan Vander Heyden
    • 1
  • Debby Mangelings
    • 1
    Email author
  1. 1.Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling (FABI)Vrije Universiteit Brussel (VUB)BrusselsBelgium

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