Speciation analysis of arsenic in samples containing high concentrations of chloride by LC-HG-AFS
Chloride widely exists in the environment and will cause serious interference for arsenic speciation analysis. The determination of four arsenic species including arsenite (As(III)), arsenate (As(V)), monomethylarsenate (MMA), and dimethylarsonate (DMA) in samples containing high concentrations of Cl− was carried out in this work by coupling of liquid chromatography (LC) with hydride generation atomic fluorescence spectrometry (HG-AFS). The interference of Cl− was successfully eliminated by coupling two anion-exchange chromatographic columns in series and eluting with 35.0 mmol L−1 (NH4)2HPO4 (pH = 6.00). A novel pre-treatment system was subsequently developed to realize on-line column switch and pre-reduction of As(V). The analysis time was shortened by an isocratic elution but programmed flow rate method, and the sensitivity of As(V) was also enhanced by the introduction of pre-reduction using the developed system. The proposed method can resist at least 10 g L−1 Cl− without any pre-treatment operations. Since LC-HG-AFS is low-cost and can be afforded or self-assembled by most labs, the developed method can be adopted as a routine analysis method for arsenic species in chloride-bearing samples, such as urine and seawater.
KeywordsArsenic Speciation analysis Chloride LC-HG-AFS
This work was supported by the National Natural Science Foundation of China (grant numbers U1607129, U1607123) and the Yangtze Scholars and Innovative Research Team of the Chinese University (grant number IRT_17R81).
Compliance with ethical standards
The Certified Reference Material for arsenic speciation analysis in urine (GBW09115) was obtained from the National Standard Substances Centre in China, and this study did not involve any animal or human participants. The authors declare that all experiments were performed in accordance with the relevant laws and ethical standards.
Conflict of interest
The authors declare that they have no competing interests.
- 5.Doker S, Yilmaz M. Speciation of arsenic in spring, well, and tap water by high-performance liquid chromatography-inductively coupled plasma-mass spectrometry. Anal Lett. 2018;51:254–64.Google Scholar
- 10.Diacomanolis V, Noller BN, Taga R, Harris HH, Aitken JB, Ng JC. Relationship of arsenic speciation and bioavailability in mine wastes for human health risk assessment. Environ Chem. 2016;13:641–55.Google Scholar
- 13.Jackson BP, Liba A, Nelson J. Advantages of reaction cell ICP-MS on doubly charged interferences for arsenic and selenium analysis in foods. J Anal Atom Spectrom. 2015;30:1179–83.Google Scholar
- 15.Castillo A, Boix C, Fabregat N, Roig-Navarro AF, Rodriguez-Castrillon JA. Rapid screening of arsenic species in urine from exposed human by inductively coupled plasma mass spectrometry with germanium as internal standard. J Anal Atom Spectrom. 2012;27:354–8.Google Scholar
- 18.Lin CH, Chen Y, Su YA, Luo YT, Shih TT, Sun YC. Nanocomposite-coated microfluidic-based photocatalyst-assisted reduction device to couple high-performance liquid chromatography and inductively coupled plasma-mass spectrometry for online determination of inorganic arsenic species in natural water. Anal Chem. 2017;89:5892–900.Google Scholar
- 19.Schmidt L, Landero JA, Santos RF, Mesko MF, Mello PA, Flores EMM, et al. Arsenic speciation in seafood by LC-ICP-MS/MS: method development and influence of culinary treatment. J Anal Atom Spectrom. 2017;32:1490–9.Google Scholar
- 20.Guimaraes D, Roberts AA, Tehrani MW, Huang R, Smieska L, Woll AR, et al. Characterization of arsenic in dried baby shrimp (Acetes sp.) using synchrotron-based X-ray spectrometry and LC coupled to ICP-MS/MS. J Anal Atom Spectrom. 2018;33:1616–30.Google Scholar
- 21.Quarles CD, Sullivan P, Field MP, Smith S, Wiederin DR. Use of an inline dilution method to eliminate species interconversion for LC-ICP-MS based applications: focus on arsenic in urine. J Anal Atom Spectrom. 2018;33:745–51.Google Scholar
- 22.Grijalba AC, Fiorentini EF, Martinez LD, Wuilloud RGA. Comparative evaluation of different ionic liquids for arsenic species separation and determination in wine varietals by liquid chromatography - hydride generation atomic fluorescence spectrometry. J Chromatogr A. 2016;1462:44–54.Google Scholar
- 23.Liu CX, Xiao ZM, Jia Z, Tian J, Liu XL, Fan X. Quantitative determination of arsenic species in feed using liquid chromatography-hydride generation atomic fluorescence spectrometry. Chin J Anal Chem. 2018;46:537–42.Google Scholar
- 25.Currier JM, Saunders RJ, Ding L, Bodnar W, Cable P, Matousek T, et al. Comparative oxidation state specific analysis of arsenic species by high-performance liquid chromatography-inductively coupled plasma-mass spectrometry and hydride generation-cryotrapping-atomic absorption spectrometry. J Anal Atom Spectrom. 2013;28:843–52.Google Scholar
- 27.Florez MR, Garcia-Ruiz E, Bolea-Fernandez E, Vanhaecke F, Resano M. A simple dilute-and-shoot approach for the determination of ultra-trace levels of arsenic in biological fluids via ICP-MS using CH3F/He as a reaction gas. J Anal Atom Spectrom. 2016;31:245–51.Google Scholar
- 28.Yu XP, Deng TL, Guo YF, Wang Q. Arsenic species analysis in freshwater using liquid chromatography combined to hydride generation atomic fluorescence spectrometry. J Anal Chem. 2014;69:83–8.Google Scholar
- 29.Pinheiro FC, Amaral CDB, Schiavo D, Nobrega JA. Determination of arsenic in fruit juices using inductively coupled plasma tandem mass spectrometry (ICP-MS/MS). Food Anal Methods. 2017;10:992–8.Google Scholar
- 30.Amaral CDB, Dionisio AGG, Santos MC, Donati GL, Nobrega JA, Nogueira ARA. Evaluation of sample preparation procedures and krypton as an interference standard probe for arsenic speciation by HPLC-ICP-QMS. J Anal Atom Spectrom. 2013;28:1303–10.Google Scholar
- 31.An J, Lee H, Nam K, Yoon HO. Effect of methanol addition on generation of isobaric polyatomic ions in the analysis of arsenic with ICP-MS. Microchem J. 2017;131:170–3.Google Scholar
- 34.Liu Q, Lu XF, Peng HY, Popowich A, Tao J, Uppal JS, et al. Speciation of arsenic – a review of phenylarsenicals and related arsenic metabolites. Trac-Trend Anal Chem. 2018;104:171–82.Google Scholar
- 35.Sadee B, Foulkes ME, Hill SJ. Coupled techniques for arsenic speciation in food and drinking water: a review. J Anal Atom Spectrom. 2015;30:102–18.Google Scholar
- 36.McSheeh S, Mester Z. Arsenic speciation in marine certified reference materials - part 1. Identification of water-soluble arsenic species using multidimensional liquid chromatography combined with inductively coupled plasma, electrospray and electrospray high-field asymmetric waveform ion mobility spectrometry with mass spectrometric detection. J Anal Atom Spectrom. 2004;19:373–80.Google Scholar
- 37.Musil S, Matoušek T. On-line pre-reduction of pentavalent arsenicals by thioglycolic acid for speciation analysis by selective hydride generation-cryotrapping-atomic absorption spectrometry. Spectrochim Acta B. 2008;63:685–91.Google Scholar
- 40.Samanta G, Chowdhury UK, Mandal BK, Chakraborti D, Sekaran NC, Tokunaga H, et al. High performance liquid chromatography inductively coupled plasma mass spectrometry for speciation of arsenic compounds in urine. Microchem J. 2000;65:113–27.Google Scholar
- 41.An J, Lee J, Lee G, Nam K, Yoon HO. Combined use of collision cell technique and methanol addition for the analysis of arsenic in a high-chloride-containing sample by ICP-MS. Microchem J. 2015;120:77–81.Google Scholar
- 42.Zhang Q, Minami H, Inoue S, Atsuya I. Differential determination of trace amounts of arsenic(III) and arsenic(V) in seawater by solid sampling atomic absorption spectrometry after preconcentration by coprecipitation with a nickel-pyrrolidine dithiocarbamate complex. Anal Chim Acta. 2004;508:99–105.Google Scholar
- 43.dos Santos QQ, Silva MM, Lemos VA, Ferreira SLC, de Andrade JB. An online preconcentration system for speciation analysis of arsenic in seawater by hydride generation flame atomic absorption spectrometry. Microchem J. 2018;143:175–80.Google Scholar
- 44.Den SJ, Wei WC, Mierzwa J, Yang MH. On-line determination of arsenic species in seawater by selective hydride generation coupled with inductively coupled plasma-mass spectrometry. J Chin Chem Soc-Taip. 2002;49:197–205.Google Scholar