Analytical and Bioanalytical Chemistry

, Volume 411, Issue 27, pp 7251–7260 | Cite as

Speciation analysis of arsenic in samples containing high concentrations of chloride by LC-HG-AFS

  • Xiaoping YuEmail author
  • Wanjing Cui
  • Qin Wang
  • Yafei Guo
  • Tianlong DengEmail author
Research Paper


Chloride widely exists in the environment and will cause serious interference for arsenic speciation analysis. The determination of four arsenic species including arsenite (As(III)), arsenate (As(V)), monomethylarsenate (MMA), and dimethylarsonate (DMA) in samples containing high concentrations of Cl was carried out in this work by coupling of liquid chromatography (LC) with hydride generation atomic fluorescence spectrometry (HG-AFS). The interference of Cl was successfully eliminated by coupling two anion-exchange chromatographic columns in series and eluting with 35.0 mmol L−1 (NH4)2HPO4 (pH = 6.00). A novel pre-treatment system was subsequently developed to realize on-line column switch and pre-reduction of As(V). The analysis time was shortened by an isocratic elution but programmed flow rate method, and the sensitivity of As(V) was also enhanced by the introduction of pre-reduction using the developed system. The proposed method can resist at least 10 g L−1 Cl without any pre-treatment operations. Since LC-HG-AFS is low-cost and can be afforded or self-assembled by most labs, the developed method can be adopted as a routine analysis method for arsenic species in chloride-bearing samples, such as urine and seawater.

Graphical abstract


Arsenic Speciation analysis Chloride LC-HG-AFS 


Funding information

This work was supported by the National Natural Science Foundation of China (grant numbers U1607129, U1607123) and the Yangtze Scholars and Innovative Research Team of the Chinese University (grant number IRT_17R81).

Compliance with ethical standards

The Certified Reference Material for arsenic speciation analysis in urine (GBW09115) was obtained from the National Standard Substances Centre in China, and this study did not involve any animal or human participants. The authors declare that all experiments were performed in accordance with the relevant laws and ethical standards.

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

216_2019_2093_MOESM1_ESM.pdf (283 kb)
ESM 1 (PDF 283 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Tianjin Key Laboratory of Brine Chemical Engineering and Resource Eco-utilizationTianjin University of Science and TechnologyTianjinPeople’s Republic of China

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