Analytical and Bioanalytical Chemistry

, Volume 411, Issue 27, pp 7055–7059 | Cite as

Simultaneous determination of proteins in microstructured optical fibers supported by chemometric tools

  • Natalia A. BurmistrovaEmail author
  • Pavel S. Pidenko
  • Sergei A. Pidenko
  • Yulia S. Skibina
  • Yulia B. Monakhova


A new perspective on the relevant problem—creating simple, rapid, and efficient protein sensors based on microstructured optical fibers using a simple homogeneous analysis format—was proposed. Commercially available long-period grating hollow core microstructured optical fibers (LPG HCMOF) were used to determine bovine serum albumin (BSA) and albumin from chicken eggs (OVA) in binary mixtures as well as immunoglobulin G (IgG) in the presence of BSA and OVA. LPG HCMOF transmission spectra allowed the detection of both BSA and OVA up to 10 mg/mL with LOD as low as 0.1 and 0.8 μg/mL, respectively. Partial least squares regression (PLS) was utilized for modeling of LPG HCMOF spectral data and quantitative analysis of BSA, OVA, total protein, and IgG in binary and ternary mixtures. Rather high coefficients of determination (R2) and low root mean square error for the calibration (RMSEC) (15%) and prediction (RMSEP) (20%) were obtained for all PLS models. The proposed approach was tested in the analysis of BSA in spiked horse blood hemolyzed (HBH). The results demonstrated the functionality of the proposed approach and offered the opportunity for the creation of a wide range of sensors for protein determination in complex mixtures.

Graphical abstract


Microstructured optical fibers Long-period grating fiber Protein determination Chemometrics Partial least squares regression 


Funding information

The reported study was funded by the Russian Foundation for Basic Research according to the research project № 18-29-08033 and Russian Ministry of Science and Education, project 4.1063.2017/4.6.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2019_2085_MOESM1_ESM.pdf (690 kb)
ESM 1 (PDF 690 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Natalia A. Burmistrova
    • 1
    Email author
  • Pavel S. Pidenko
    • 1
  • Sergei A. Pidenko
    • 1
  • Yulia S. Skibina
    • 2
  • Yulia B. Monakhova
    • 1
    • 3
  1. 1.Institute of ChemistrySaratov State UniversitySaratovRussia
  2. 2.SPE LLC Nanostructured Glass TechnologySaratovRussia
  3. 3.Spectral Service AGCologneGermany

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