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Analytical and Bioanalytical Chemistry

, Volume 411, Issue 26, pp 6813–6823 | Cite as

Self-heating tilted fiber Bragg grating device for melt curve analysis of solid-phase DNA hybridization and thermal cycling

  • Jason Koppert
  • Hubert Jean-Ruel
  • Devin O’Neill
  • Chris Harder
  • William Willmore
  • Anatoli Ianoul
  • Jacques AlbertEmail author
Paper in Forefront

Abstract

We demonstrate a DNA-based optical fiber device that uses an in-fiber grating, a light absorbing coating with surface anchored DNA, and a built-in optical thermometer. This device is used for precisely thermal cycling surface DNA spots bound by a simple UV cross-linking technique. Near-infrared light of wavelengths near 1550 nm and guided power near 300 mW is coupled out of the fiber core by a tilted fiber Bragg grating inscribed in the fiber and absorbed by the coating to increase its temperature to more than 95 °C. A co-propagating broadband light signal (also in the near-infrared region) is used to measure the reflection spectrum of the grating and thus the temperature from the wavelength shifts of the reflection peaks. The device is capable of sensitive DNA melt analysis and can be used for DNA amplification.

Graphical abstract

Keywords

DNA Fiber grating PCR Fiber sensor 

Notes

Acknowledgements

This work was funded by grants from the Natural Sciences and Engineering Research Council of Canada and Spartan Biosciences Inc. Gratings were fabricated in the Advanced Photonics Laboratory of Carleton University by Albane Laronche.

Compliance with ethical standards

This research does not involve any human participants or animals.

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

216_2019_2072_MOESM1_ESM.pdf (798 kb)
ESM 1 (PDF 798 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of ElectronicsCarleton UniversityOttawaCanada
  2. 2.Spartan Biosciences Inc.NepeanCanada
  3. 3.Department of ChemistryCarleton UniversityOttawaCanada
  4. 4.Department of BiochemistryCarleton UniversityOttawaCanada

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