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A label-free photoelectrochemical DNA biosensor using a quantum dot–dendrimer nanocomposite

  • Faten DivsarEmail author
Research Paper

Abstract

A novel label-free photoelectrochemical biosensing method for highly sensitive and specific detection of DNA hybridization using a CdS quantum dot (QD)–dendrimer nanocomposite is presented. A molecular beacon (MB) was assembled on a gold-nanoparticle-modified indium tin oxide electrode surface. Hybridization to a complementary target DNA disrupts the stem–loop structure of the MB, which was afterward labeled with the QD–dendrimer nanocomposite. The modified indium tin oxide electrode showed a stable anodic photocurrent response at 300 mV (vs Ag/AgCl) to light excitation at 410 nm in the presence of 0.1 M ascorbic acid as an electron donor. The protocol developed integrates the specificity of an MB for molecular recognition and the advantages of gold nanoparticles for increasing the loading capacity of the MB on the electrode surface and accelerating the electron transfer. Moreover, the photocurrent was greatly enhanced because of the high loading of QDs by the dendrimer, which eliminated the surface defects of CdS QDs and prevented recombination of their photogenerated electron–hole pairs. Under the optimal conditions, a linear relationship between the increase of photocurrent and target DNA concentration was obtained in the range from 1 fM to 0.1 nM, with a detection limit of 0.5 fM. The sequence-specificity experiment showed that one or three mismatches of DNA bases could be discriminated. This photoelectrochemical method is a prospective technique for DNA hybridization detection because of its great advantages: label-free, high sensitivity and specificity, low cost, and easy fabrication. This could create a new platform for the application of CdS QD–dendrimer nanocomposites in photoelectrochemical bioanalysis.

Graphical abstract

Keywords

Poly(amidoamine) dendrimer Quantum dots Nanocomposites Photoelectrochemical biosensor 

Notes

Compliance with ethical standards

Conflict of interest

The author declare that she has no competing interests.

Supplementary material

216_2019_2058_MOESM1_ESM.pdf (393 kb)
ESM 1 (PDF 393 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of ChemistryPayame Noor UniversityTehranIran

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