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Fluctuation correlation spectroscopy and its applications in homogeneous analysis

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Abstract

Fluctuation correlation spectroscopy (FCS) is a single-molecule/particle detection technique based on measuring signal fluctuations in a highly focused detection volume. Multiple-parameter information can be obtained from the FCS measurement including the amplitude, characteristic diffusion time of correlation curve, and brightness of the adopted probes. The multiple-parameter change is related with physical or chemical change occurring in the probes. Meanwhile, the detection method has advantages such as short sample time in seconds, sample volume with low limit in femtoliters, and mixing to detection without any separations. These advantages make the FCS technique suitable for homogeneous analysis. In this review, we summarized recent novel applications of FCS and its variants in homogeneous analysis including nucleic acid analysis, protein analysis, enzyme activity assay, direct characterization of nanoparticles in solution, and others.

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Funding

Project funded by China Postdoctoral Science Foundation (Grant number 2018 M640379). This work was sponsored by Shanghai Sailing Program from Shanghai Committee of Science and Technology (Grant number 19YF1422000). This work was also financially supported by the NSFC (Grant Nos. 21327004, 21475087, 21675111 and 81670308).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China

    Di Su, Chaoqing Dong & Jicun Ren

  2. Shanghai University of Medicine & Health Sciences Affiliated Sixth People’s Hospital South Campus, 6600 Nanfeng Highway, Shanghai, 201499, China

    Di Su & Yuemei Hou

  3. State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China

    Jicun Ren

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  1. Di Su
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Published in the topical collection Young Investigators in (Bio-)Analytical Chemistry with guest editors Erin Baker, Kerstin Leopold, Francesco Ricci, and Wei Wang.

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Su, D., Hou, Y., Dong, C. et al. Fluctuation correlation spectroscopy and its applications in homogeneous analysis. Anal Bioanal Chem 411, 4523–4540 (2019). https://doi.org/10.1007/s00216-019-01884-1

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