Microporous polymer based on the new compound “bi-(4-vinyl phenylquinoline) amide” for enrichment and quantitative determination of lamotrigine in rat and human serum
Lamotrigine is one of the most widely used antiepileptic drugs in the treatment of epilepsy. This kind of drug needs to be used in the long term and should be quantitatively detected in the blood of patients to avoid drug toxicity caused by individual differences and environmental and pathological changes in the process of taking. The detection of antiepileptic drugs in human blood is challenging because of their low contents and the interference of complex matrices. Thus, the sample enrichment method has been commonly used to improve the sensitivity of detection. In this work, we have synthesized a new “bi-(4-vinyl phenylquinoline) amide” compound and used it as the monomer to produce the hyper-cross-linked microporous polymer for the enrichment of lamotrigine. This material has a high adsorption capacity, specificity, and linearity, which can improve the detection sensitivity of lamotrigine by high-performance liquid chromatography (HPLC). The mechanism of this phenomenon has also been investigated. Finally, we have developed the microporous polymer enrichment coupled with HPLC method for the quantitative determination of lamotrigine in rat and human serum samples. This method has excellent precision, accuracy, and recovery, meeting the test of biological sample. The low limit of quantitation was 0.625 μg/mL.
KeywordsMicroporous polymer material Solid-phase extraction Lamotrigine High-performance liquid chromatography
This study was supported by the National Natural Science Foundation of China (No. 21505116). The work was also supported by the Jiangsu Natural Science Foundation of China (Nos. BK20171183 and BK20181147), the Postdoctoral Foundation of Jiangsu Province (No. 1701132C), the scientific research funds of talents from Xuzhou Medical University (No. D2015014), and the Natural Science Foundation of Xuzhou City (Nos. KC17187, KC17102, and KC18050).
Compliance with ethical standards
Informed consent was obtained from all individual participants included in the study and the experiments were approved by the ethics committee.
Conflict of interest
The authors declare that they have no conflict of interest.
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