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Prêt-à-porter nanoYESα and nanoYESβ bioluminescent cell biosensors for ultrarapid and sensitive screening of endocrine-disrupting chemicals

  • Antonia Lopreside
  • Maria Maddalena Calabretta
  • Laura Montali
  • Maura Ferri
  • Annalisa Tassoni
  • Bruce R. Branchini
  • Tara Southworth
  • Marcello D’Elia
  • Aldo Roda
  • Elisa MicheliniEmail author
Research Paper
  • 39 Downloads
Part of the following topical collections:
  1. Young Investigators in (Bio-)Analytical Chemistry

Abstract

Cell-based assays utilizing reporter gene technology have been widely exploited for biosensing, as they provide useful information about the bioavailability and cell toxicity of target analytes. The long assay time due to gene transcription and translation is one of the main drawbacks of cell biosensors. We report the development of two yeast biosensors stably expressing human estrogen receptors α and β and employing NanoLuc as the reporter protein to upgrade the widely used yeast estrogen screening (YES) assays. A viability control strain was also developed based on a chimeric green-emitting luciferase, PLG2, expressed for the first time in Saccharomycescerevisiae. Thanks to their brightness, NanoLuc and PLG2 provided excellent sensitivity, enabling the implementation of these biosensors into low-cost smartphone-based devices. The developed biosensors had a rapid (1 h) response and reported on (anti)estrogenic activity via human estrogen receptors α and β as well as general sample toxicity. Under optimized conditions, we obtained LODs of 7.1 ± 0.4 nM and 0.38 ± 0.08 nM for E2 with nanoYESα and nanoYESβ, respectively. As a proof of concept, we analyzed real samples from plants showing significant estrogenic activity or known to contain significant amounts of phytoestrogens.

Graphical abstract

Keywords

Bioluminescence Biosensor Luciferase Smartphone Estrogen receptor 

Notes

Acknowledgements

We wish to thank Dr. Alessandro Filippini and Dr. Dario Zanichelli (Phenbiox Srl, Bologna, Italy) for providing soybean seed extracts, and Dr. Matteo Lamborghini (Alfavita Srl, Ravenna, Italy) for providing alfalfa plant extracts.

Funding information

This research was sponsored in part by PRIN 2015 “Multifunctional nanotools for advanced cancer diagnostics” (Prot. 2015TWP83Z), PRIN 2015 “Securing and ensuring sustainable use of agriculture waste, co-and by-products: an integrated analytical approach combining mass spectrometry with health effect-based biosensing” (Prot. 2015FFY97L), the NATO Science for Peace and Security Programme under Grant No. 985042, Air Force Office of Scientific Research (FA9950-18-1-0017) and National Science Foundation (MCB-1410390).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2019_1805_MOESM1_ESM.pdf (381 kb)
ESM 1 (PDF 381 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Antonia Lopreside
    • 1
  • Maria Maddalena Calabretta
    • 1
  • Laura Montali
    • 1
  • Maura Ferri
    • 2
    • 3
  • Annalisa Tassoni
    • 2
  • Bruce R. Branchini
    • 4
  • Tara Southworth
    • 4
  • Marcello D’Elia
    • 5
  • Aldo Roda
    • 1
    • 6
  • Elisa Michelini
    • 1
    • 6
    • 7
    Email author
  1. 1.Department of Chemistry “G. Ciamician”University of BolognaBolognaItaly
  2. 2.Department of Biological Geological and Environmental Sciences (BIGeA)University of BolognaBolognaItaly
  3. 3.Department of Civil, Chemical, Environmental and Materials EngineeringUniversity of BolognaBolognaItaly
  4. 4.Department of ChemistryConnecticut CollegeNew LondonUSA
  5. 5.Gabinetto Regionale di Polizia Scientifica per l’Emilia-RomagnaBolognaItaly
  6. 6.INBB, Istituto Nazionale di Biostrutture e BiosistemiRomeItaly
  7. 7.Health Sciences and Technologies-Interdepartmental Center for Industrial Research (HST-ICIR)University of BolognaOzzano dell’EmiliaItaly

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