Determination of sulfonamides in meat with dummy-template molecularly imprinted polymer-based chemiluminescence sensor

  • Zhao Bin Li
  • Jing Liu
  • Ju Xiang Liu
  • Zhan Hui Wang
  • Jian Ping WangEmail author
Research Paper


In this study, a molecularly imprinted polymer capable of recognizing 15 sulfonamides was first synthesized with sulfabenz as the dummy template. The calculation results from computation simulation showed that the specific 3D conformation of the template had an important influence on the polymer’s recognition ability. Then, the polymer was used as recognition reagent to prepare a chemiluminescence sensor on a conventional 96-well microplate for the determination of the residues of 15 sulfonamides in meat (chicken and pork). Due to the 4-(imidazol-1-yl)phenol-enhanced luminol–H2O2 system, the limits of detection for the 15 analytes were in the range of 1.0–12 pg/mL. The recoveries from the standard fortified blank samples were in the range of 72.7–99%. Furthermore, one assay could be finished within 30 min, and the sensor could be reused 4 times. Therefore, this sensor could be used as a very useful tool for routine screening of residues of sulfonamides in meat samples.

Graphical abstract

Assay procedures of the molecularly imprinted polymer-based chemiluminescence sensor for determination of sulfonamides.


Sulfabenz Sulfonamides Computational simulation Molecularly imprinted polymer Chemiluminescence sensor 


Funding information

The authors are grateful for the financial support from Hebei Key Research and Development Project (18226628D), Hebei Layer/Broiler Industry Technology System (HBCT2018150210), and Beijing Municipal Natural Science Foundation (6182019).

Compliance with ethical standards

This article does not contain any studies with humans or animals performed by any of the authors.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Zhao Bin Li
    • 1
  • Jing Liu
    • 1
  • Ju Xiang Liu
    • 1
  • Zhan Hui Wang
    • 2
  • Jian Ping Wang
    • 1
    Email author
  1. 1.College of Veterinary MedicineAgricultural University of HebeiBaodingChina
  2. 2.College of Veterinary MedicineChina Agricultural UniversityBeijingChina

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