Characterization of the chemical composition of Drimia numidica plant parts using high-resolution mass spectrometry: study of their total phenolic content and antioxidant activity

  • Eleni Kakouri
  • Charalabos Kanakis
  • Panayiotis Trigas
  • Petros A. TarantilisEmail author
Research Paper


Drimia species have been used since ancient times for their medicinal properties. Their bulbs are considered as the main source of secondary metabolites with biological activity but the chemical composition of the other plant parts has not yet been considered. The aim of this study is to contribute to the existing knowledge with new data on the total phenolic content, the antioxidant activity and the chemical profile of different parts of Drimia numidica. The total phenolic content was estimated by the Folin-Ciocalteu assay and the antioxidant activity with DPPH· and ABTS·+ reagents. The separation and the identification of the compounds were performed with liquid chromatography combined with time-of-flight high-resolution mass spectrometry (LC/Q-TOF/HRMS). The extract of leaves presented the highest phenolic content while the highest antioxidant activity was presented by the extract of flowers. Results of the chemical analysis verify the presence of bufadienolides and phenolic compounds.

Graphical abstract


Drimia numidica LC/Q-TOF/HRMS Antioxidant activity Phenolic compounds Bufadienolides 



The authors want to thank Dr. Georgios Danezis for his valuable mentoring and support regarding the Folin-Ciocalteu validation experiments.

Compliance with ethical standards

This article does not contain any studies involving animals performed by any of the authors.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2019_1781_MOESM1_ESM.pdf (3.9 mb)
ESM 1 (PDF 3.92 mb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Eleni Kakouri
    • 1
  • Charalabos Kanakis
    • 1
  • Panayiotis Trigas
    • 2
  • Petros A. Tarantilis
    • 1
    Email author
  1. 1.Laboratory of Chemistry, Department of Food Science & Human Nutrition, School of Food Biotechnology and DevelopmentAgricultural University of AthensAthensGreece
  2. 2.Laboratory of Systematic Botany, Department of Crop ScienceAgricultural University of AthensAthensGreece

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