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Development of a heart-cutting supercritical fluid chromatography-high-performance liquid chromatography coupled to tandem mass spectrometry for the determination of four tobacco-specific nitrosamines in mainstream smoke

  • Yan Zhou
  • Haobo Zhang
  • Xianying Wang
  • Dawei QiEmail author
  • Wenbo GuEmail author
  • Da Wu
  • Baizhan Liu
Research Paper

Abstract

This paper proposed a newly developed heart-cutting two-dimensional supercritical fluid chromatography-high-performance liquid chromatography system coupled to tandem mass spectrometry (SFC-HPLC-MS/MS) for the determination of four tobacco-specific nitrosamines (TSNAs) in cigarette mainstream smoke. The orthogonality of five SFC columns and two HPLC columns was evaluated. The 1-AA column was applied for the first dimensional (1D) SFC separation to isolate the target compounds from the complex cigarette smoke matrices, and a trapping column in conjunction with an isocratic pump was employed to capture the 1D elutes. Then, the trapped 1D elutes were transferred into the C18 column through a two-position/six-port valve for the second dimensional (2D) analysis. The ion suppression was significantly reduced by the established SFC-HPLC system; meanwhile, the matrix interferences were eliminated as the results demonstrated. A dynamic range between 0.1 and 20 ng/mL was achieved with LOQs of 0.72 μg/cig for N-nitrosonornicotine (NNN), 0.66 μg/cig for nicotine-derived nitrosamine ketone (NNK), 0.81 μg/cig for N-nitrosoanatabine (NAT), and 0.39 μg/cig for N-nitrosoanabasine (NAB). All the results revealed that the presented method exhibited good repeatabilities and recoveries and could be used as a rapid and reliable approach for routine analysis of TSNAs in mainstream smoke.

Keywords

Two-dimensional Supercritical fluid chromatography High-performance liquid chromatography Tobacco-specific nitrosamines Mainstream smoke 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2019_1746_MOESM1_ESM.pdf (200 kb)
ESM 1 (PDF 200 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Technical Center of Shanghai Tobacco Group Co. Ltd.ShanghaiChina

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